Relationship between DNA replication and DNA repair in human lymphocytes proliferating in vitro in the presence and in absence of mutagen

• Autorzy: Szyfter K , Wiktorowicz K. , Wielgosz M.S. , Zajaczek S. , Kujawski M. , Jaloszynski P. , Czub M. , Markowska J.
• Języki publikacji: EN

Abstrakty

EN

The effects of mutagens on DNA replication and DNA repair were studied in peripheral blood lymphocytes (PBL) obtained from 21 healthy subjects, 2 samples from healthy heterozygote of Xeroderma pigmentosum (XP) and 2 samples from patient with clinically recognised XP. Inter-individual variations were found in DNA replication and in the level of spontaneous DNA repair measured under standard culture condition. Exposure of human PBL proliferating in vitro to B(a)P was followed by a partial inhibition of replicative DNA synthesis in all subjects and by an induction of DNA repair in healthy subjects. In XP patients DNA repair synthesis remained at the level attributed to spontaneous DNA repair. The response to mutagen varied individually. Results were analysed statistically. It was established that the studied indices of DNA synthesis correlate well with each other. The highest correlation was found between the levels of spontaneous and B(a)P-induced DNA repair. It is concluded that the level of spontaneous DNA repair is predictive for an estimation of cells ability to repair DNA damage. Inter-individual variations in the inhibition of DNA replication and in DNA repair synthesis are also dependent on the type of mutagen as shown by effects of other mutagens. Different effects of mutagen exposure on the inhibition of DNA replicative synthesis and induction of DNA repair can be explained by genetically controlled differences in the activity of enzymes responsible for mutagen processing and lesion removal.

Słowa kluczowe

EN

genotoxicity; DNA replication; human lymphocyte; DNA repair; lymphocyte proliferation; mutagenesis; in vitro

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1995
• Tom: 36
• Numer: 4
• Opis fizyczny: p.379-388,fig.

Twórcy

autor

Szyfter K

• Polish Academy of Sciences, 60-479 Poznan, Strzeszynska 34, Poland

autor

Wiktorowicz K.

autor

Wielgosz M.S.

autor

Zajaczek S.

autor

Kujawski M.

autor

Jaloszynski P.

autor

Czub M.

autor

Markowska J.

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