Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. I. The technology for the preparation of the primers

• Autorzy: Borowicz B P
• Języki publikacji: EN

Abstrakty

EN

The above mentioned technology in a case of the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene is described i.e. the primers design, purification and phosphorylation of the oligonucleotide primers.

PL

Dla określonego w tytule pracy, celu badań przedstawiono technologię przygotowywania starterów, w przypadku DNA odpowiadającego Otwartej Ramie Odczytu II genu I-18 C Chironomus tentans. Technologia ta obejmowała zaprojektowanie starterów dla PCR oraz oczyszczenie i fosforylację zsyntetyzowanych oligonukleotydowych starterów.

Słowa kluczowe

EN

phosphorylation; plant protection; I-18 C gene; purification; Chironomus tentans; polymerase chain reaction; DNA fragment; oligonucleotide

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 1997
• Tom: 37
• Numer: 1-2
• Opis fizyczny: p.138-144

Twórcy

autor

Borowicz B P

• Institute of Plant Protection, Miczurina 20, 60-318 Poznan, Poland

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