Isolation of the freshwater Naegleria fowleri [Carter, 1970] from a river using a monoclonal antibody and the polymerase chain reaction

• Autorzy: Sparagano O , Revol A.
• Języki publikacji: EN

Abstrakty

EN

ELISA and Southern blots techniques were employed to detect the pathogenic amoeba Naegleria fowleri in river water used for cooling by a French power plant. Water samples from 100 ml to 1 ml, collected at two sites, were filtered. After incubation of the filters at 44°C for 48 h, 21 Naegleria strains were removed. 4 of those strains were identified as N. fowleri by both ELISA and DNA probe, showing the good correlation between these techniques applied to environmental samples.

PL

Zastosowano metodę ELISA oraz technikę „Southern blot” do wykrycia chorobotwórczej ameby Naegleria fowleri w wodzie rzecznej używanej do chłodzenia w francuskiej elektrowni. Próby wody (1, 10, 100 ml) pobierano na 2 stanowiskach i filtrowano. Po inkubacji sączków w temp. 44°C przez 48 h wykryto 21 szczepów Naegleria. Cztery z nich zidentyfikowano jako Naegleria fowleri zarówno przy pomocy testu ELISA jak i badania DNA, co wskazuje na dużą zbieżność wyników tych metod stosowanych do prób środowiskowych.

Słowa kluczowe

EN

monoclonal antibody; fresh water; isolation; amoeba; ELISA test; Naegleria fowleri; polymerase chain reaction; hydrobiology; DNA probe

• Wydawca: -
• Czasopismo: Acta Hydrobiologica
• Rocznik: 1995
• Tom: 37
• Numer: 1
• Opis fizyczny: p.29-32,fig.

Twórcy

autor

Sparagano O

• The University of Reading, School of Animal and Microbial Sciences, Whiteknights, PO Box 228, Reading RG6, 2AJ, U.K.

autor

Revol A.

Bibliografia

• Flores B.M., Garcia C.A., Stamm W.E. and Torian B.E. 1990. Differentiation of Naegleria fowleri from Acanthamoeba species by using monoclonal antibodies and flow cytometry. J. Clin. Microbiol., 28, 1999-2005.
• Hu W.N., Kopachik W. and Band R.N. 1992. Cloning and characterization of transcripts showing virulence-related gene expression in Naegleria fowleri. Infec. Immun., 60, 2418-2424.
• Jadin J.B. 1975. Amibes "limax" vecteurs possibles de mycobactéries et de Mycobacterium leprae. Acta Leprol., 59/60, 157-167.
• John D.T. 1982. Primary amoebic meningocephalitis and the biology of Naegleria fowleri. Ann. Rev. Microbiol., 36, 101-123.
• Marciano-Cabral F. 1988. Biology of Naegleria spp. Microbiol. Rev., 52, 114-133.
• Martinez A.J. 1983. Free-living amoebae pathogenic aspects. A review. Protistol. Abstracts, 7, 293-306.
• Picard C., Ponsonnet C., Paget E., Nesme X. and Simonet P. 1992. Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction. Appl. Environ. Microbiol., 58, 2717-2722.
• Rowbotham T.J. 1983. Isolation of Legionella pneumophila from clinical specimens via amoebae and the interaction of those and other isolates with amoebae. J. Clin. Pathol., 36, 978-986.
• Sparagano O. 1993. Differentiation of Naegleria fowleri and other Naegleriae by polymerase chain reaction and hybridization methods. FEMS Microbiol. Lett., 110, 325-330.
• Sparagano O., Drouet E., Brebant R., Manet E., Denoyel G. and Pernin P. 1993. Use of monoclonal antibodies to distinguish pathogenic Naegleria fowleri (cysts, trophozoites, or flagellate forms) from other Naegleria species. J. Clin. Microbiol., 31, 2758-2763.
• Sparagano O., Drouet E., Denoyel G. and Pernin P. 1994. Polymerase chain reaction to detect the pathogenic Naegleria fowleri: application to water samples. J. Microbiol. Meth., 19, 81-88.
• Van Belkum A., De Jonckheere J.K. and Quint W.G.V. 1992. Genotyping Naegleria spp. and Naegleria fowleri isolates by interrepeat polymerase chain reaction. J. Clin. Microbiol., 30, 2595-2598.
• Visvesvara G.S., Peralta M.J., Brandt F.H., Wilson M., Aloisio C. and Franko E. 1987. Production of monoclonal antibodies to Naegleria fowleri, agent of primary amoebic meningocephalitis. J. Clin. Microbiol., 25, 1629-1634.