Modulation of inflammatory response by pentoxifylline is independent of heme oxygenase-1 pathway

• Autorzy: Taha H. , Grochot-Przeczek A. , Was H. , Kotlinowski J. , Kozakowska M. , Marek A. , Skrzypek K. , Lackowska B. , Balcerczyk A. , Mustafa S. , Dulak J. , Jozkowicz A.
• Języki publikacji: EN

Abstrakty

EN

Objective: It was reported that some effects of pentoxifylline (PTX) are mediated by heme oxygenase-1 (HO-1) induction. We investigated the role of HO-1 in anti-inflammatory activity of PTX. Methods: Experiments were performed in human and murine monocytes and endothelial cells and in HO-1 deficient mice. Results: PTX dose-dependently decreased expression of HO-1 in cell lines studied. As expected, PTX reduced also production of TNF. This effect was independent of HO-1 activity, as demonstrated in cells treated with HO-1 activators and inhibitors or in cells overexpressing HO-1. Moreover, inhibition of TNF was the same in human endothelial cells of different HO-1 genotypes, showing that PTX is similarly efficient in carriers of more and less active HO-1 promoter variants. In mice, PTX did not influence HO-1 expression, as measured in liver, kidney, spleen, heart, and skin. Accordingly, the response of PTX treated animals to LPS was the same in wild type and HO-1 deficient mice. PTX to a similar extent increased influx of leukocyte into peritoneal cavity, decreased production of TNF and reduced expression of VCAM-1 in vascular intima. Conclusion: PTX inhibits production of TNF and may decrease inflammatory reaction both in vitro and in vivo, but these effects are independent of HO-1.

Słowa kluczowe

EN

macrophage; endothelial cell; antiinflammatory activity; mouse; heme oxygenase-1; modulation; cytokine; knockout mice; inflammation; pentoxifylline; monocyte; inflammatory response; mice; knockout mouse

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2009
• Tom: 60
• Numer: 2
• Opis fizyczny: p.3-12,fig.,ref.

Twórcy

autor

Taha H.

• Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland

autor

Grochot-Przeczek A.

autor

Was H.

autor

Kotlinowski J.

autor

Kozakowska M.

autor

Marek A.

autor

Skrzypek K.

autor

Lackowska B.

autor

Balcerczyk A.

autor

Mustafa S.

autor

Dulak J.

autor

Jozkowicz A.