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2003 | 50 | 1 |

Tytuł artykułu

Defective brca2 influences topoisomerase I activity in mammalian cells

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The Chinese hamster cell mutant V-C8 is defective in the Brca2 gene (Kraakman-van der Zwet et al., 2002, Cell Biol.-, 22: 669). Here we report that V-C8 cells were 10-fold more sensitive to camptothecin, an inhibitor of topoisomerase I, than the parental V79 cells. The level of the relaxation activity of topoisomerase I in nuclear extracts was also lower (4-fold) in V-C8 than V79 cells, in spite of the fact that the level of the topoisomerase I protein was the same in these cells. The survival of V-C8 cells in the presence of camptothecin, the sensitivity of V-C8 topoisomerase I to camptothecin, and the level of the relaxation activity in V-C8 nuclear extract were almost completely restored by transfection of V-C8 cells with the murine Brca2 gene or by the transfer of human chromosome 13 providing the BRCA2 gene. These results indicate that the ob­served changes in the topoisomerase I activity in V-C8 are due to the defective func­tion of the Brca2 gene.

Wydawca

-

Rocznik

Tom

50

Numer

1

Opis fizyczny

p.139-144,fig.

Twórcy

  • Medical University of Warsaw, S.Banacha 1, 02-097 Warsaw, Poland
autor
autor

Bibliografia

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Bibliografia

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