Manipulating freezing tolerance in transgenic plants

• Autorzy: McKersie B D , Murnaghan J. , Bowley S.R.
• Języki publikacji: EN

Abstrakty

EN

Winterhardiness is a composite of tolerances to freezing, desiccation, ice-encasement, flooding and diseases. From one point of view, winterhardiness may not be easily manipulated by genetic engineering technology because many different genes are involved in the tolerance of these diverse stresses. However, these various stresses have similarities. They promote formation of activated forms of oxygen, promote membrane lipid and protein degradation, cause similar biophysical changes in membrane structure, and culminate with increased leakage of cytoplasmic solutes and loss of cellular membrane functions. These similarities led to the hypothesis that winter injury might be reduced in crop plants if their tolerance of oxidative stress was increased. Towards that objective we created transgenic alfalfa (Medicago sativa L.) plants that overexpress either Mn-SOD or Fe-SOD cDNA (provided by Dirk Inzé, Universiteit Gent). Petiole explants were transformed using Agrobacterium tumefaciens and plants were regenerated by somatic embryogenesis. The primary transgenic plants were screened using PCR (polymerase chain reaction), Southern hybridization and native PAGE for SOD activity. Greenhouse and laboratory studies showed a minimal difference in stress tolerance between the primary transgenic and non-transgenic plants. In the first field trial, four primary transgenic plants expressing two forms of the Mn-SOD cDNA had greater survival after two winters than the non-transgenic RA3. Similar results were obtained in a second field trial, comparing 18 independent transformants with Mn-SOD targeted to the mitochondria, 11 independent transformants with Mn-SOD targeted to the chloroplast and 39 independent transformants with Fe-SOD targeted to the chloroplast, expressed in three different non-transgenic plants. The transgenic plants averaged over 25% higher survival than the non-transgenic controls after one winter. There was no effect of subcellular targeting or SOD type on field survival, but there was variation among independent transformants containing the same SOD construct. Activated oxygen therefore appears to be one of the possible causes of winter injury, and it should be possible to reduce winter injury in transgenic plants by constitutive overexpression of SOD.

Słowa kluczowe

EN

flood; superoxide dismutase; disease; alfalfa; freezing tolerance; Agrobacterium tumefaciens; transgenic plant; embryogenesis; hardiness; plant; anoxia; Medicago sativa

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 1997
• Tom: 19
• Numer: 4
• Opis fizyczny: p.485-495,fig.

Twórcy

autor

McKersie B D

• University of Guelph, Guelph, ON, Canada

autor

Murnaghan J.

autor

Bowley S.R.

Bibliografia

• Andrews C.J., Pomeroy M.K. 1989. Physiological properties of plants affecting ice-encasement tolerance. Icel Agr Sci 2: 41–51.
• Baker C.J., Orlandi E.W. 1995. Active oxygen in plant pathogenesis. Annu Rev Phytopathol 33: 299–321.
• Bannister J.V., Bannister W.H., Rotils G. 1987. Aspects of the structure, function and applications of superoxide dismutase. CRC Crit Reviews Biochem 22: 110–180.
• Bhaumik G., Srivastava K.K., Selvamurthy W., Purkayastha S.S. 1995. The role of free radicals in cold injuries. Int J Biometeorol 38: 171–175.
• Borochov A., Walker M.A., Kendall E.J., Pauls K.P., McKersie B.D. 1987. Effect of a freeze-thaw cycle on properties of microsomal membranes from wheat. Plant Physiol 84: 131–134.
• Bowler C., Alliotte T., De Loose M., Van Montagu M., Inzé D. 1989. The induction of manganese superoxide dismutase in response to stress in Nicotiana plumbaginifolia. EMBO J. 8: 31–38.
• Bowler C., Van Camp W., Van Montagu M., Inzé D. 1994. Superoxide dismutase in plants. Crit Reviews Plant Sci 13: 199–218.
• Bowler C., Van Montagu M., Inzé D. 1992. Superoxide dismutase and stress tolerance. Annu Rev Plant Physiol Plant Mol Biol 43: 83–116.
• Bowler C., Slooten L., Vandenbranden S, De Rycke R., Botterman J., Sybesma C., Van Montagu M., Inzé D. 1991. Manganese superoxide dismutase can reduce cellular damage mediated by oxygen radicals in transgenic plants. EMBO J 10: 1723–1732.
• Bowley S.R., Kielly G.A., Anandarajah K., McKersie B.D., Senaratna T. 1993. Field evaluation following two cycles of backcross transfer of somatic embryogenesis to commercial alfalfa germplasm. Can J Plant Sci 73: 131–137.
• Bridger G.M., Yang W., Falk D.E., McKersie B.D. 1995. Cold acclimation increases tolerance of activated oxygen in winter cereals. J Plant Physiol.
• Chia L.S., McRae D.G., Thompson J.E. 1982. Light-dependence of paraquat-initiated membrane deterioration in bean plants. Evidence for the involvement of superoxide. Physiol Plant 56: 492–499.
• Chen Y. 1993. Improvement of stress tolerance in alfalfa Medicago sativa L. by genetic engineering. PhD thesis, University of Guelph.
• D’Halluin K., Botterman J., De Greef W. 1990. Engineering of herbicide-resistant alfalfa and evaluation under field conditions. Crop Sci 30: 866–871.
• De Beus, M.D. 1991. Evaluation of transgenic alfalfa Medicago sativa L. for enhanced stress tolerance. MSc thesis, University of Guelph.
• Elstner E.F. 1982. Oxygen activation and oxygen toxicity. Annu Rev Plant Physiol 33: 73–96.
• Elstner E.F. 1991. Mechanisms of oxygen activation in different compartments of plant cells. In Active oxygen/oxidative stress and plant metabolism, EJ Pell, KL Steffen eds, American Soc. Plant Physiology. Rockville, M.D., pp. 13–25.
• Fowler D.B., Lumin A.E., Gusta L.V. 1983. Breeding for winterhardiness in wheat. In New Frontiers in Winter Wheat Production., D. B. Fowler, L. V. Gusta, A. E. Slinkard, B. A. Hobin eds, University of Saskatchewan, Saskatoon, Canada., pp. 136–184.
• Foyer C.H., Descourvieres P., Kunert K.J. 1994. Protection against oxygen radicals: an important defence mechanism studied in transgenic plants. Plant Cell Env 17: 507–523.
• Grant M.N. 1983. Winter wheat breeding objectives for Western Canada. In New Frontiers in Winter Wheat Production., D.B. Fowler, L. V. Gusta, A. E. Slinkard, B. A. Hobin eds, University of Saskatchewan, Saskatoon, Canada., pp. 89–101.
• Gutteridge J.C., Halliwell B. 1990. The measurement and mechanism of lipid peroxidation in biological systems. Trends Biochem Sci 15: 129–135.
• Halliwell B. 1987a. Oxidants and human desease: some new concepts. FASEB J 1: 358.
• Halliwell B. 1987b. Oxidative damage, lipid peroxidation and anti-oxidant protection in chloroplasts. Chem Phys Lipids 44: 327–340.
• Halliwell B., Gutteridge J. 1984. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J 219: 1–14.
• Hetherington P.R., Broughton H.L., McKersie B.D. 1988. Ice-encasement injury to microsomal membranes from winter wheat crowns II. Changes in membrane lipids during ice-encasement. Plant Physiol 86: 740–743.
• Hetherington P.R., McKersie B.D., Borochov A. 1987. Ice-encasement injury to microsomal membranes from winter wheat crowns I. Comparison of membrane properties after lethal ice-encasement and during a postthaw period. Plant Physiol 85: 1068–1072.
• Jiang C., Iu B., Singh J. 1996. Requirement of a CCGAC cis-acting element for cold induction of the BN115 gene from winter Brassica napus. Plant Mol Biol 30: 679–684.
• Kendall E.J., McKersie B.D. 1989. Free radical and freezing injury to cell membranes of winter wheat. Physiol Plant 76: 86–94.
• Kendall E.J., McKersie B.D., Stinson RH. 1985. Phase properties of membranes after freezing injury in winter wheat. Can J Bot 63: 2274–2277.
• Lawrence K., Bhalla P, Misra P.C. 1995. NADH-dependent redox activities on the external face of plasma membrane vesicles of chickpea roots. J Plant Physiol 146: 763–765.
• Levine A., Tenhaken R., Dixon R., Lamb C. 1994. H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell 79: 583–593.
• Low P.S., Merida J.R 1996. The oxidative burst in plant defense: Function and signal transduction. Physiol Plant 96: 533–542.
• Lynch D.V., Steponkus P.L. 1987. Plasma membrane lipid alterations associated with cold acclimation of winter rye seedlings Scale cereale L. cv. Puma. Plant Physiol 83: 761–767.
• Marcotte W.R. Jr., Russell S.H., Quatrano R.S. 1989. Abscisic acid-responsive sequences from the Em gene of wheat. Plant Cell 1: 969–976.
• McKenzie J.S., Paquin R., Duke S.H. 1988. Cold and heat tolerance. In Alfalfa and Alfalfa Improvement. AA Hanson, DK Barnes, RR Hill, eds. American Society of Agronomy, Madison, WI. Pp259–302.
• McKersie B.D., Bowley S.R. 1993. Synthetic seeds in alfalfa. In Synseeds Applications of Synthetic Seeds to Crop Improvement., K. Redenbaugh ed, CRC Press, Boca Raton., pp. 231–255.
• McKersie B.D., Chen Y.R., de Beus M., Bowley S.R., Bowler C., Inzé D., D’Halluin K., Botterman J. 1993. Superoxide dismutase enhances tolerance of freezing stress in transgenic alfalfa Medicago sativa L.. Plant Physiol 103: 1155–1163.
• McKersie B.D., Bowley S.R., Harjanto E., Leprince O. 1996. Water deficit tolerance and field performance of transgenic alfalfa overexpressing superoxide dismutase. Plant Physiol 111: 1177–1181.
• McKersie B.D., Leshem Y.Y. 1994. Stress and stress coping in cultivated plants. Kluwer Academic Publishers, Dordrecht, Netherlands.
• McKersie B.D., Thompson J.E. 1978. Phase behavior of chloroplast and microsomal membranes during [kidney beans] leaf senescence. Plant Physiol 61: 639–643.
• Morre D.J., Brightman A.O., Hidalgo A., Navas P. 1995. Selective inhibition of auxin-stimulated NADH oxidase activity and elongation growth of soybean hypocotyls by thiol reagents. Plant Physiol 107: 1285–1291.
• Morre D.J., Navas P., Penel C., Castillo F.J. 1986. Auxin stimulated NADH oxidase semihydroascorbate reductase of soybean plasma membrane: role in acidification of cytoplasm. Protoplasma 133: 195–197.
• Otter T., Polle A. 1994. The influence of apoplastic ascorbate on the activities of cell wall-associated peroxidase and NADH oxidase in needles of Norway spruce Picea abies L. Plant Cell Physiol 35: 1231–1238.
• Pukacki P.M., Kendall E.J. McKersie B.D. 1991. Membrane injury during freezing stress to winter wheat Triticum aestivum L. crowns. J Plant Physiol 138: 516–521.
• Scandalios J. G. 1990. Response of plant antioxidant defense genes to environmental stress. Adv Genet 28: 1–41.
• Scandalios J.G. 1993. Oxygen stress and superoxide dismutases. Plant Physiol 101: 7–12.
• Senaratna T., McKersie B.D., Borochov A. 1987. Desiccation and free radical mediated changes in plant membranes. J Exptl Bot 38: 2005–2014.
• Senaratna T., McKersie B.D., Stinson R.H. 1984. Association between membrane phase properties and dehydration injury in soybean axes. Plant Physiol 76: 759–762.
• Schubert B.K. 1994. Glutathione, glutathione reductase and freezing stress in alfalfa Medicago sativa L. MSc thesis, University of Guelph.
• Uemura M., Steponkus P.L. 1994. A contrast of the plasma membrane lipid composition of oat and rye leaves in relation to freezing tolerance. Plant Physiol 104: 479–496.