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2004 | 51 | 3 |

Tytuł artykułu

Sulforaphane-mediated induction of a phase 2 detoxifying enzyme NAD[P]H: quinone reductase and apoptosis in human lymphoblastoid cells

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The effect of sulforaphane on human lymphoblastoid cells originating from a patient of a high cancer risk was studied. Sulforaphane (SFN) is a naturally occurring substance of chemopreventive activity. In our study, changes in cell growth, induc­tion of apoptosis and phase 2 enzymes as well as glutathione level were examined. Apoptosis was tested by confocal microscopy at three stages: change in mitochon­drial membrane potential, caspase activation and phosphatidylserine externalization. We show that SFN increases the activity of the detoxification system: it in­creases quinone reductase activity at low concentration (0.5-1 uM) and raises glutathione level in a dose-dependent manner. At higher doses (2.5-10 uM) sulforaphane is a cell growth modulator, as it caused cell growth cessation (IC50 = 3.875 uM), and apoptosis inducer. The results obtained suggest that sulforaphane acts as a chemopreventive agent in human lymphoblastoid cells.

Wydawca

-

Rocznik

Tom

51

Numer

3

Opis fizyczny

p.711-721,fig.,ref.

Twórcy

autor
  • National Institute of Public Health, Warsaw, Poland
autor
autor
autor

Bibliografia

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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-9e6f0679-3c13-4e71-b6e0-d830a4a9f860
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