Platelet activating factor [PAF] inhibitor [TCV-309] reduces caerulein - and PAF-induced pancreatitis. A morphologic and functional study in the rat

• Autorzy: Tomaszewska R , Dembinski A. , Warzecha Z. , Banas M. , Konturek S.J. , Stachura J.
• Języki publikacji: EN

Abstrakty

EN

Caerulein-induced acute pancreatitis was studied in rats. Consistent with this type of acute pancreatitis morphological (edema, leukocytic infiltration and acinar cell vaculization) and biochemical (increase in pancreatic protein content, PAF release and serum amylase) changes developed 5 hours after caerulein administration. In addition increase in pancreatic weight and decrease in pancreatic blood flow were noticed. PAF administration caused pancreatic damage similar in some parameters to caerulein-induced pancreatitis, along with reduction of pancreatic blood flow, increase in pancreatic protein content, and serum amylase. TCV-309, a selective PAF antagonist, administered prior to caerulein and/or PAF, reduced caerulein-induced pancreatitis and prevented PAF-induced pancreatitis. Results of our present studies indicate the crucial role of PAF in pathogenesis of experimental acute pancreatitis.

Słowa kluczowe

EN

platelet-activating factor; acute pancreatitis; caerulein; pancreatitis; blood flow; rat

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 1992
• Tom: 43
• Numer: 4
• Opis fizyczny: p.345-352,fig.

Twórcy

autor

Tomaszewska R

• University Medical School, 31-531 Krakow, Grzegorzecka 16, Poland

autor

Dembinski A.

autor

Warzecha Z.

autor

Banas M.

autor

Konturek S.J.

autor

Stachura J.

Bibliografia

• 1. Lampel M, Kern HF. Acute interstitial pancreatitis in the rat induced by excessive doses of a pancreatic secreatogogue. Virchows Arch Path Anat Histol 1977; 373: 97-117.
• 2. Adler D, Hupp T, Kern HF. Course and spontaneous regression of acute pancreatitis in rats. Virchows Arch Path Anat Histol 1979; 31: 282-294.
• 3. Robert A, Lum JT, Lancaster C, Olafsson AS, Kolbasa KP, Nezamis JE. Prevention by prostaglandins of caerulein-induced pancreatitis in rats. Lab Invest 1989; 60: 677-691.
• 4. Soling H-D, Fest W. Synthesis of l-o-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor) in exocrine glands and its control by secreatagogues. J Biol Chem 1986; 261: 13916-13922.
• 5. Wallace JL, Steel G, Whittle BJR, Lagente V, Vargafting B. Evidence for platelet-activating factor as a mediator of endotoxin-induced gastrointestinal damage in the rat. Gastroenterology 1987; 93: 765-773.
• 6. Emanuelli G, Montucchio G, Gaia E, Dughera L, Corvetti G, Gubetta L. Experimental acute pancreatitis induced by platelet activating factor in rabbits. Am J Pathol 1989; 134: 315-326.
• 7. Bernfeld P. Amylase alfa and beta. Methods in Enzymology 1955; 5: 139-148.
• 8. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with Folin phenol reagent. J Biol Chem 1951; 193: 265-275.
• 9. Eliakim R, Karmeli F, Razin E, Rachmilewitz D. Role of platelet-activating factor in ulcerative colitis. Gastroenterology 1988; 95: 1167-1172.
• 10. Konturek SJ, Dembiński A, Warzecha Z, Pawlik W, Gustaw P, Stachura J, Tomaszewska R, Żmuda A. Role of platelet activating factor in pathogenesis of acute pancreatitis. Gut 1992; 33: 1268-1274.
• 11. Watanabe O, Baccino FM, Steer ML, Meldolesi J. Supramaximal caerulein stimulation and ultrastructure of rat pancreatic acinar cell: early morphological changes during development of experimental pancreatitis. Am J Physiol 1984; 246: G457-467.
• 12. Dąbrowski A, Gabryelewicz A, Chyczewski L. The Effect of Platelet Activating Factor Antagonist (BN52021) on caerulein-induced acute pancreatitis with reference to oxygen radicals. Int J Pancreatol 1991; 8: 1-11.