Fagocytoza i zabijanie komorek Staphylococcus aureus przez granulocyty myszy poddanych dzialaniu stymulatora kolonii granulocytarno-makrofagowych [GM-CSF]

• Autorzy: Czygier M. , Mroczko B. , Szmitkowski M. , Jankowski D.
• Języki publikacji: PL

Abstrakty

PL

GM-CSF należy do stymulatorów hematopoezy, działających na komórki macierzyste ukierunkowane. Stymuluje głównie komórki wspólne dla linii granulocytów i makrofagów. Istnieją doniesienia, że podobnie jak inne cytokiny hematopoetyczne działa na funkcje dojrzałych komórek w warunkach in vitro. W niniejszej pracy wykazano wpływ GM-CSF na funkcje fagocytarne, właściwości bakteriobójcze i aktywność enzymów lizosomalnych granulocytów w warunkach żywego organizmu myszy.

EN

GM-CSF is a hematopoietic growth factor. In vitro it stimulates the proliferation of myeloid progenitors and formation of granulocyte and macrophage colonies. It was found that GM-CSF in vitro is also stimulated the function of mature granulocytes, but we have no information about such influence in vivo. The purpose of this investigation was the evaluation in vivo of the GM-CSF effect on phagocytosis, bactericidal activity, and lysosome enzyme activities in granulocytes. GM-CSF was injected into mice subcutaneously during 5 consecutive days in the dose of 1 µg/kg/d. The examination of the percent of cell phagocytising bacteria (Staphylococcus aureus), NBT test, bactericidal activity and activation of acid phosphatase, alkaline phosphatase, peroxidase and esterase was performed every day and an evident increase of the tested parmeters was found. These results prove in vivo activation of granulocytes by GM-CSF.

Słowa kluczowe

PL

wlasciwosci bakteriobojcze; makrofagi; zwierzeta laboratoryjne; aktywnosc enzymatyczna; stymulatory hematopoezy; fagocytoza; bakterie; cytokiny; Staphylococcus aureus

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 1998
• Tom: 50
• Numer: 3-4
• Opis fizyczny: s.151-160,wykr.,bibliogr.

Twórcy

autor

Czygier M.

• Akademia Medyczna, 15-176 Bialystok, ul.M.Sklodowskiej-Curie 24a

autor

Mroczko B.

autor

Szmitkowski M.

autor

Jankowski D.

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