Candida albicans Berthout, 1923: Hydrolases activity and own method of digestive tract strains biotyping

• Autorzy: Kurnatowski M , Wasowska-Krolikowska K. , Kurnatowska A.
• Języki publikacji: EN

Abstrakty

EN

112 strains of Candida albicans were isolated from oral cavity and ontocenoses of the upper digestive tract (endoscopy) of children (age: 5-17) with gastrointestinal disorders. Axenic strains were differentiated with API 20C AUX and API ZYM tests (bioMerieux). Then enzymograms and biotypes were determined for all the strains based on the activity of 19 hydrolases. The highest activity was noted for: e₂ - phosphatase alcaline, e₆ – leucine arylamidase, e₁₁ - phosphatase acid, e₅ - lipase (c₁₄); e₇ - valine arylamidase, e₁₂ - naphtol-AS-BI-phosphohydrolase, e₁₆ - α-glucosidase and e₁₈ - N-acetyl-ß-glucosamidase, and the latter four were used for biotyping procedures. Our own system was based on the mathematical binominal distribution formula (1: 4: 6: 4: 1): all „+”; one „ -” three „+”; two „-”, two ,,+”; tbree „-”, one „+”; all „-”. We bave found the following biotypes: A (16.1 ± 3.5%), B₁ (2.7 ± 2.53%), B₃ (8.0 ± 25%), B₄ (22.3 ± 3.9%), C₂ (1.8 ± 1.3%), C₃ (7.1 ± 2.4%), C₆ (30.4 ± 4.3%), D₃ (11.6 ± 3.0%).

Słowa kluczowe

EN

strain; gastrointestinal tract; hydrolase activity; biotype; Candida albicans

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2002
• Tom: 48
• Numer: 4
• Opis fizyczny: p.441-445,fig.,ref.

Twórcy

autor

Kurnatowski M

• Medical University, Sporna 36-50, 91-738 Lodz, Poland

autor

Wasowska-Krolikowska K.

autor

Kurnatowska A.

Bibliografia

• Kurnatowska A. 1971. Problem of multifocal infection by Candida, Geotrichum, Rhodotorula and Torulopsis in women. Comptes-Rendus 1. Multicolloque Europeen de Parasitolgie, 517-518.
• Kurnatowska A. 1995. Wybrane zagadnienia mikologii medycznej. Promedi, Łódź.
• Kurnatowska A. J., Kurnatowski P. 1998. Biotypes of fungi isolated from patients with oral cavity diseases. Mikologia Lekarska 5: 213-217.
• Kurnatowski M., Wąsowska-Królikowska K., Kurnatowska A. 1999. Biotypes of fungi isolated from children wilh alimentary tract diseases. Mycoses 4: 189.
• Willliamson M.J., Samaranayake L., Macfarlane T.W. 1986. Biotypes of oral Candida albicans and Candida tropicalis isolates. Journal of Medicine and Veterinary Mycology 24: 81-84.