Effects of DAGO on the rodent hippocampal evoked potentials using different perfusion solutions

• Autorzy: SanMartin S , Menendez L. , Gutierrez M. , Hidalgo A. , Baamonde A.
• Języki publikacji: EN

Abstrakty

EN

Opioid receptor agonists exert excitatory effects in the hippocampus by inhibiting GABA release. We report that the |Li-opioid agonist, DAGO, increases the amplitude of the population spikes (PS) measured in the stratum pyramidale of the CA1 cell layer in mouse and rat hippocampal slices perfused with an artificial cerebrospinal fluid (ACSF), but not when perfused in Krebs solution. The GABAa agonist, 3-APS, induces inhibitory responses when perfused in either ACSF or Krebs. Also, the field excitatory postsynaptic potentials (EPSP) measured on stratum radiatum do not differ when the slice is perfused with either ACSF or Krebs. The increase in the amplitude of the PS induced by DAGO is not obtained when perfused in a modified ACSF whose concentration of MgS04 was lowered to its concentration in the Krebs solution (from 2.4 mM to 1.2 mM). Thus, changes in the concentration of MgS04 seem to be responsible for the different responses induced by DAGO.

Słowa kluczowe

EN

mice; drug; excitatory effect; electrophysiological recording; electrical stimulation; opioid receptor; gamma-aminobutyric acid; magnesium sulphate; artificial cerebrospinal fluid; population spike; mouse; excitatory postsynaptic potential; hippocampus

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2000
• Tom: 60
• Numer: 2
• Opis fizyczny: p.187-193,fig.

Twórcy

autor

SanMartin S

• Laboratorio de Farmacologia, C-Julian Claveria s-n, 33006 Oviedo, Spain

autor

Menendez L.

autor

Gutierrez M.

autor

Hidalgo A.

autor

Baamonde A.

Bibliografia

• Bostock E., Dingledine R., Xu G., Chang K.-J. (1984) Mu opioid receptors participate in the excitatory effect of opi­ates in the hippocampal slice. J. Pharmacol. Exp. Ther. 231 : 512-517.
• Capogna M., Gahwiler B.H., Thompson S.M. (1993) Mech­anism of jLi-opioid receptor-mediated presynaptic inhibi­tion in the rat hippocampus in vitro. J. Physiol. (Lond) 470: 539-558.
• Dingledine R. (1981) Possible mechanism of enkephalin ac­tion on hippocampal CA1 pyramidal neurons. J. Neurosci. 1: 1022-1035.
• Dunwiddie T., Mueller A., Palmer M., Stewart J., Hoffer B. (1980) Electrophysiological interactions of enkephalins with neuronal circuitry in the rat hippocampus. I. Effects on pyramidal cell activity. Brain Res. 184: 311-330.
• Fleck M.W., Henze D.A., Barrionuevo G., Palmer A.M. (1993) Aspartate and glutamate mediate excitatory synap­tic transmission in area CA1 of the hippocampus. J. Neu­rosci. 13: 3944-3955.
• Fueta Y., Siniscalchi A., Tancredi V., Avoli M. (1995) Extrac­ellular magnesium and anticonvulsant effects of valproate in young rat hippocampus. Epilepsia 36: 404-409.
• Lupica C.R., Dunwiddie T.V. (1991) Differential effects of mu- and delta-receptor selective opioid agonists on feed­forward and feedback GAB Aergic inhibition in hippocam­pal brain slices. Synapse 8: 237-246.
• Mansour A., Fox C.A., Thompson R.C., Akil H., Watson S.J. (1994) jn-opioid receptor mRNA expression in the rat CNS: comparison to ^-receptor binding. Brain Res. 643: 245-265.
• Rodriguez F.D., Bardaji E., Traynor J.R. (1992) Differential effects of Mg+2 and other divalent cations on the binding of tritiated opioid ligand. J. Neurochem. 59: 462-472.
• SanMartin S., Andres-Trelles F., Menendez L., Meana A., Hi­dalgo A., Baamonde A. (1996) Interaction among alfaxa- lone, pregnenolone sulfate, and two GABAa agonists on hippocampal slices. Cell Mol. Neurobiol. 16: 427-431.
• SanMartin S., Menendez L., Gutierrez M., Hidalgo A., Baamonde A. (1999) Effects of diethylstilbestrol on mouse hippocampal evoked potentials in vitro. Cell Mol. Neuro­biol. 19:691-703.
• Tejwani G.A., Hanissian S.H. (1990) Modulation of mu, delta and kappa opioid receptors in rat brain by metal ions and histidine. Neuropharmacology 29: 445-452.
• Valentino R.J., Dingledine R. (1982) Pharmacological char­acterization of opioid effects in the rat hippocampal slice. J. Pharmacol. Exp. Ther. 223: 502-509.
• Zhang A., Fan S.-H.,-Cheng T. P.-O., Altura B.T., Wong R.K.S., Altura M.B. (1996) Extracellular Mg+2 modulates intracellular Ca+2 in acutely isolated hippocampal CA1 py­ramidal cells of the guinea-pig. Brain Res. 728: 204-208.
• Zieglgansberger W., French E.D., Siggins G.R., Bloom F.E. (1979) Opioid peptides may excite hippocampal pyramidal neurons by inhibiting adjacent inhibitory interneurons. Science 205:415-417.