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2008 | 64 | 04B |

Tytuł artykułu

Kriokonserwacja nasienia maskulinizowanych samic pstraga teczowego

Warianty tytułu

EN
Cryopreservation of sperm of rainbow trout neomales

Języki publikacji

PL

Abstrakty

EN
The objectives of this study were: sperm cryopreservation, computer assisted sperm motility analysis (CASA) and improvement of sperm motility through in vitro incubation of rainbow trout neomale spermatozoa. In vitro incubation of sperm in ASP (Artificial Seminal Plasma) solution resulted in an increase of the percentage of motile spermatozoa, while other sperm motility characteristics remained unchanged. The highest hatching rates were obtained for sperm cryopreserved in straws (16.3% and 25.0%): cryopreservation in pellets was less successful (7.7% and 3.8%), for non-incubated and incubated sperm, respectively. There were significant regressions between hatching rates and straight-line velocity (VSL; R² = 0.75) and average path velocity (VAP; R² = 0.74) for sperm preincubated in ASP and cryopreserved in straws. These data indicate that sperm of rainbow trout neomales may be successfully cryopreserved and CASA analysis is useful for the prediction of fertilizing ability of cryopreserved sperm.

Wydawca

-

Rocznik

Tom

64

Numer

04B

Opis fizyczny

s.613-616,rys.,tab.,bibliogr.

Twórcy

autor
  • Instytut Rozrodu Zwierzat i Badan Zywnosci PAN, ul.Tuwima 10, 10-747 Olsztyn
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Bibliografia

  • 1. Billard R., Takashima F.: Resorption of spermatozoa in the sperm duct of rainbow trout during the post-spawning period. Bull. Jpn. Soc. Sci. Fish. 1983, 49, 387-392.
  • 2. Dietrich G. J., Kowalski R., Wojtczak M., Dobosz S., Goryczko K., Ciereszko A.: Motility parameters of rainbow trout (Oncorhynchus mykiss) spermatozoa in relation to sequential collection of milt, time of post-mortem storage and anesthesia. Fish Physiol. Biochem. 2005, 31, 1-9.
  • 3. Donaldson E. M.: Manipulation of reproduction in farmed fish. Anim. Reprod. Sci. 1996, 42, 381-392.
  • 4. Geffen A. J., Evans J. P.: Sperm traits and fertilization success of male and sex-reversed female rainbow trout (Oncorhynchus mykiss). Aquaculture 2000, 182, 61-72.
  • 5. Glogowski J., Kwasnik M., Piros B., Dabrowski K., Goryczko K., Dobosz S., Kuzminski H., Ciereszko A.: Characterization of rainbow trout milt collected with a catheter: semen parameters and cryopreservation success. Aquac. Res. 2000, 31, 289-296.
  • 6. Goryczko K.: Pstrągi, chów i hodowla. Poradnik hodowcy.: Wyd. IRŚ, Olsztyn 2005.
  • 7. Morisawa S., Morisawa M.: Acquisition of potential for sperm motility in rainbow trout and chum salmon. J. Exp. Biol. 1986, 126, 89-96.
  • 8. Pandian T. J., Koteeswaran R.: Ploidy induction and sex control in fish. Hydrobiologia 1998, 384, 167-243.
  • 9. Piferrer F., Donaldson E. M.: Sex control in Pacific salmon, [w:] Bromage N., Donaldson E. M., Zanuy S., Carrilo M., Planas J.: Applications of Comparative Endocrinology to Fish Culture, [w:] Muir J. F., Roberts R. J. (wyd.): Recent Advances in Aquaculture IV. Blackwell, Oxford 1993, 69-77.
  • 10. Robles V., Cabrita E., Cuńado S., Herraez M. P.: Sperm cryopreservation of sex-reversed rainbow trout (Oncorhynchus mykiss): parameters that affect its ability for freezing. Aquaculture 2003, 224, 203-212.
  • 11. Rurangwa E., Volckaert F. A. M., Huyskens G., Kime D. E., Ollevier F.: Quality control of refrigerated and cryopreserved semen using computer-assisted sperm analysis (CASA), viable staining and standardized fertilisation in African catfish (Clarias gariepinus). Theriogenology 2000, 55, 751-769.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-9a713d84-6141-4830-af2d-d6472eda45f2
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