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2006 | 09 | 2 |

Tytuł artykułu

The hemagglutinating activity of Pseudomonas aeruginosa strains isolated from humans and animals

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The presence and type of adhesins occurring in Pseudomonas aeruginosa strains were determined by hemagglutination test with a 3% suspension of normal and trypsin-treated human group A erythrocytes, with or without the addition of sugar inhibitors (D-mannose, D-glucose, L-fucose, D-galactose, D-fructose, lactose, N-acetylneuraminic acid, N-acetylglucosamine and N-acetylgalactosamine). This study showed that a low percentage of Pseudomonas aeruginosa strains caused the agglutination of normal erythrocytes. Trypsin treatment of erythrocytes did not affect the hemagglutinating properties, indicating that hemagglutination was not dependent upon a trypsin-sensitive protein on the erythrocytes surface. Most of the studied strains agglutinated RBCs at 37°C. A great variability in the inhibiting activity on studied strains was observed among the carbohydrates tested. These results demonstrated the predominant role of N-acetylglucosamine, N-acetylgalactosamine and N-acetylneuraminic acid for Pseudomonas aeruginosa adhesion to RBCs.

Wydawca

-

Rocznik

Tom

09

Numer

2

Opis fizyczny

p.121-126,ref.

Twórcy

autor
  • University of Podlasie, Prusa 12, 08-110 Siedlce, Poland
autor
autor

Bibliografia

  • Chiarini F, Girmenia C, Segnati L, Superti F, Orsi N (1987) Adhesive properties of Pseudomonas aeruginosa isolated from urinary tract infections. Boll 1st Sieroter Milan 66: 23-30.
  • Garber N, Guempel U, Belz A, Gilboa-Garber N, Doyle RJ (1992) On the specificity of the D-galactose-binding lec­tin (PA-I) of Pseudomonas aeruginosa and its strong binding to hydrophobic derivatives of D-galactose and thiogalactose. Biochim Biophys Acta 1116: 331-333.
  • Gilboa-Garber N (1972) Inhibition of broad spectrum hema­gglutinin from Pseudomonas aeruginosa by D-galactose and ist derivatives. FEBS Lett 20: 242-244.
  • Gilboa-Garber N, Sudakevitz D (1999) The hemagglutinat- ing activities of Pseudomonas aeruginosa lectins PA-IL and PA-IIL exhibit opposite temperature profiles due to different receptor types. FEMS Immunol Med Microbiol 25: 365-369.
  • Gilboa-Garber N, Lerrer B, Lesman-Movshovich E, Dgani O (2005) Differential staining of Wesstern blots of human secreted glycoproteins from serum, milk, saliva, and semi­nal fluid using lectins displaying diverse sugar specifities. Electrophoresis 26: 4396-4401.
  • Glick J and Garber N (1983) The intercellular localization of Pseudomonas aeruginosa lectins. J Gen Microbiol 129: 3085-3090.
  • Glick J, Garber N, Shohet D (1987) Surface haema- gglutinating activity of Pseudomonas aeruginosa. Microbi­os 50: 69-80.
  • Izdebska-Szymona K, Laziuk D (1988) Comparision of some adhesive properties of Pseudomonas aeruginosa strains isolated from respiratory and urinary tract infections. Acta Microbiol Polon 37: 281-293.
  • Kirkeby S, Moe D (2005) Analyses of Pseudomonas aeruginosa lectin binding to a-galactosylated glycans. Curr Microbiol 50: 309-313.
  • Ko HL, Beuth J, Solter J, Schroten H, Uhlenbruck G, Pul- verer G (1987) In vitro and in vivo inhibition of lectin mediated adhesion of Pseudomonas aeruginosa by recep­tor blocking carbohydrates. Infection 15: 237-240.
  • Old DC (1972) Inhibition of the interaction between fim- brial hemagglutinins and erythrocytes by D-mannose and other carbohydrates. J Gen Microbiol 71: 149-157.
  • Old DC (1985) Haemagglutination methods in the study of Escherichia coli. In: Sussman M (ed) The virulence of Escherichia coli. Academic Press, London, pp 287-313.
  • Pal R, Ahmed H, Chatterjee BP (1987) Hemagglutination patterns of Pseudomonas aeruginosa strains of different serogroups, Habs and Fisher types. Zentralbl Bakteriol Mikrobiol Hyg 265: 299-304.
  • Rebiere-Huet J, Di Martino P, Hulen C (2004) Inhibition of Pseudomonas aeruginosa to fibronectin by PA-IL and monosaccharides: involvement of a lectin-like process. Can J Microbiol 50: 303-312.
  • Scharfman A, Van Brussel E, Houdret N, Lamblin G, Rous­sel P (1996) Interaction between glycoconjugates from human respiratory airways and Pseudomonas aeruginosa. Am J Respir Crit Med 154: 5163-5169.
  • Sudakevitz D, Levene C, Sela R, Gilboa-Garber N (1996) Differentiation between human red cells of Pk and p blood types using Pseudomonas aeruginosa PA-I lectin. Transfusion 36: 113-116.
  • Waldström T, Trust TJ (1984) Bacterial surface lectins. Med Microbiol 4: 287-334.
  • Wolska K, Jakubczak A (2004) Haemagglutinating proper­ties in Pseudomonas aeruginosa. Med Weter 60: 653- -656 (in Polish).

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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