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2002 | 07 | 2B |

Tytuł artykułu

Genetic diversity of the Novi Sad wheat Core Collection revealed microsatellites

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
In recent years, considerable emphasis has been placed on the development of microsatellites to be used for a variety of objectives. Parental genetic diversity is a crucial requisition to derive desirable and superior progenies from crossing and selection. In order to determine desirable genotypes for hybridization, 710 wheat genotypes from the Novi Sad Core Collection, originating from 38 countries, have been evaluated during the 1993-2000 period. During those seven growth seasons, 54 agronomical, morphological, physiological and other traits have been evaluated in field and controlled conditions. In each year, the field experiment comprised 3-7 replications, while for each field replication the plot size was 1.2 m2. Based on the results from this evaluation, 96 genotypes with the highest phenotypic variation for 26 of the very important traits for wheat breeding programmes in Yugoslavia and the UK, were identified for screening with microsatellites. A set of 36 microsatellite markers was used, covering all three wheat genomes and all 42 chromosomes. For the 36 microsatellites, a total of 46 loci and 366 alleles were detected, with the average number of 7.96 alleles per locus. For 35 loci, null alleles were detected. The association of microsatellite data with phenotypic data, for 6 important traits for wheat breeding (stem height, earliness, resistance to leaf rust and powdery mildew, sedimentation value and protein content), as well as the potential for their implementation in marker, assisted selection (MAS) in wheat breeding programmes for both Yugoslavia and UK are discussed.

Wydawca

-

Rocznik

Tom

07

Numer

2B

Opis fizyczny

p.685-694

Twórcy

autor
  • Institute of Field and Vegetable Crops, M.Gorkog 30, 21000 Novi Sad, Yugoslavia
autor
autor
autor
autor

Bibliografia

  • 1. Borner, A., Roder, M. and Korzun, V. Comparative molecular mapping of GA insensitive Rht loci on chromosomes 4B and 4D of common wheat (Triticum aestivum L.). Theor. Appl. Genet. 95 (1997) 1133-1137.
  • 2. Bryan, G. J., Collins, A. J., Stephenson, P., Orry, A., Smith, J. B. and Gale, M. D. Isolation and characterization of microsatellites from hexaploid bread wheat. Theor. Apll. Genet. 94 (1997) 557-563.
  • 3. Devos, K. M., Bryan, G. J., Collins A. J., Stephenson, P. and Gale M. D. Application of two microsatellite sequences in wheat storage proteins as molecular markers. Theor. Appl. Genet. 90 (1995) 247-252.
  • 4. Donini, P., Stephenson, P., Bryan, G. J . and Koebner, R. M. D. The potential of microsatellites for high throughput genetic diversity assesment in wheat and barley. Genet. Res. Crop Evol. 45 (1998) 415-421.
  • 5. Flintham, J. E., Borner, A., Worland, A. J. and Gale, M. D. Optimising wheat grain yield: Effect of Rht (gibberelin insensitive) dwarfing genes. J. Agric. Sci. 128 (l997) 11-25.
  • 6. Gale, M. D. and Youssefian, S. Dwarfing genes in wheat. In: Progress in Plant Breeding, (Russell, G. E. Ed.), Butterworths, London, (1985) 1-35.
  • 7. Li, X., Pang, C., Wu, J., Worland, T. J., Gale, M. D. and Brown, S. J. Analysis of advantages and disadvantages of different Rht dwarfing genes in winter wheat breeding. IX Inter. Wheat Gen. Symp., Saskatoon, Saskatchewan, Canada, 2 (1998) 273-276.
  • 8. McIntosh, R. A., Hart, G. E., Devos, K. M., Gale, M. D. and Rogers, W. J. Cataloque of Gene Symbols for Wheat. Wheat Gen. Symp., Saskatoon, Saskatchewan, Canada, 5 (1998) 77-78.
  • 9. Petrović, S. and Worland, A. J. Geni reduktori visine stabljike I) Determinacija prisustva u jugoslovenskim sortama pšenice. Savremena poljoprivreda 40 (1992) 81-85.
  • 10. Plaschke, J., Ganal M. W. and Roder, M. S. Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor. Appl. Genet. 91 (1995) 1001-1007.
  • 11. Prasad, M., Varshney, R. K., Roy, J. K., Balyan, H. S. and Gupta, P. K. The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. Theor. Appl. Genet. 100 (2000) 584-592.
  • 12. Rao, V. R. and Riley, K. W. The use of biotechnology for conservation and utilization of plant genetic resources. Plant Gen. Res. Newsl. 97 (1994) 3-20.
  • 13. Roder, M., Plaschke, J., Koenig, S. U., Borner, A., Sorrells, M. E, Tanksley, S. D. and Ganal, M. W. Abundance, variation and chromosomal location of microsatellites in wheat. Mol. Gen. Genet. 246 (1997) 327-333.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-9a050748-259f-4fc5-952b-5405e3ed7eba
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