Influence of N-acetylcysteine on ICAM-1 expression and IL-8 release from endothelial and epithelial cells

• Autorzy: Radomska-Lesniewska D.M. , Sadowska A.M. , Van Overveld F.J. , Demkow U. , Zielinski J. , De Backer W.A.
• Języki publikacji: EN

Abstrakty

EN

Chronic obstructive pulmonary disease (COPD) is characterized by chronic airway inflammation. The initial step in the inflammatory process is overexpression of adhesion molecules, which leads to excessive transmigration of neutrophils. One of these adhesion molecules is ICAM-1 which is elevated in COPD patients. In this study we evaluated the influence of N-acetylcysteine (NAC) (0.01 mM-30 mM) on the cytokine-induced (TNF-alphalpha/IL-1ß) expression of the ICAM-1 adhesion molecule and on IL-8 release in endothelial (ECV-304) and bronchial epithelial (H292) cell lines. The methodology used consisted of immunochemistry for the assessment of surface ICAM-1 and ELISA method for that of soluble ICAM-1 and IL-8. NAC inhibited the TNF-alphalpha/IL-1ß-stimulated ICAM-1 expression and IL-8 release from both cell lines in a concentration dependent manner. The most effective concentrations were 30 mM and 20 mM (99 and 90% inhibition respectively, P<0.01). We conclude that NAC is an effective inhibitor of TNF-alphalpha/IL-1ß- stimulated ICAM-1 and IL-8 release in endothelial and epithelial cells. This fact highlights the anti-inflammatory potential of NAC in COPD.

Słowa kluczowe

EN

interleukin 8; chronic obstructive pulmonary disease; epithelial cell; endothelial cell; N-acetylcysteine; intercellular adhesion molecule-1; chronic airway inflammation

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology. Supplement
• Rocznik: 2006
• Tom: 57
• Numer: 4
• Opis fizyczny: p.325-334,fig.,ref.

Twórcy

autor

Radomska-Lesniewska D.M.

• Institute of Tuberculosis and Lung Diseases, Plocka 26 St., 01-138 Warsaw, Poland

autor

Sadowska A.M.

autor

Van Overveld F.J.

autor

Demkow U.

autor

Zielinski J.

autor

De Backer W.A.