Application of mass spectrometry [MS] in the structural analysis od selected polypeptides of boar seminal plasma obtained after two-dimensional electrophoresis [2-D PAGE]

• Autorzy: Kordan W , Malinowska A. , Mogielnicka M. , Lecewicz M.

Warianty tytułu

PL

Zastosowanie spektrometrii mas [MS] do charakterystyki struktury wybranych polipeptydow plazmy nasienia knura uzyskanych po elektroforezie dwukierunkowej [2-D PAGE]

• Języki publikacji: EN

Abstrakty

EN

Two-dimensional electrophoresis (2-D PAGE) led to identification in the polypeptide maps of boar seminal plasma of four conserved polypeptides with identical molecular weight of 24 kDA, and different ranges of isoelectric point (pI): (1) 7.4-7.7, (2) 8.1-8.4, (3) 8.5-8.8 and (4) 9.2-9.4. In the current study the molecular structures of these polypeptides were analysed, for the first time, by mass spectrometry (LC – MS/MS). Computerized mass spectrometry analysis of the peptides obtained after trypsin-digestion of the polypeptides demonstrated their similarity to the family of spermadhesins (crystal structure of two members of the spermadhesin family), especially to epididymal spermadhesin AWN-1. In addition, homology was found of peptides 3 and 4 with a lysozyme C precursor (1,4-beta-N-acetylmuramidase C). The results presented might indicate the participation of the analysed polypeptides in the processes accompanying fertilization.

PL

Stosując metodę elektroforezy dwukierunkowej (2-D PAGE) zidentyfikowano w składzie map polipeptydowych plazmy nasienia knura cztery zakonserwowane polipeptydy o identycznej masie cząsteczkowej 24 kDa i następujących zakresach punktu izoelektrycznego: (1) 7,4-7,7; (2) 8,1-8,4;(3) 8,5-8,8 i (4) 9,2-9,4. W analizie ich struktury molekularnej zastosowano spektroskopię mas (LC – MS/MS). W prezentowanych badaniach, zastosowana po raz pierwszy komputerowa analiza widma masowego peptydów uzyskanych po trawieniu trypsyną wszystkich badanych polipeptydów, wykazała ich podobieństwo do rodziny spermadhezyn (cristal structure of two members of the spermadhesin family),zwłaszcza do spermadhezyny najądrzowej – AWN-1. Dodatkowo wykazano homologię polipeptydów 3 i 4 z prekursorem lizozymu C (1,4-beta-N-acetylmuramidase C). Prezentowane wyniki sugerować mogą udział analizowanych polipeptydów w procesach towarzyszących zapłodnieniu.

Słowa kluczowe

EN

mass spectrometry; identification; boar; polypeptide; seminal plasma; two-dimensional electrophoresis; structural analysis

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2009
• Tom: 27
• Numer: 1
• Opis fizyczny: p.41-50,fig.,ref.

Twórcy

autor

Kordan W

• University of Warmia and Mazury in Olsztyn, 10-917 Olsztyn-Kortowo, Poland

autor

Malinowska A.

autor

Mogielnicka M.

autor

Lecewicz M.

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