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2008 | 57 | 2 |

Tytuł artykułu

A comparative evaluation of PCR ribotyping and ERIC PCR for determining the diversity of clinical Pseudomonas aeruginosa isolates

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Two typing methods were evaluated, utilizing 62 clinical strains of Pseudomonas aeruginosa, to assess their usefulness as tools to study the bacterial diversity within this complex group. Genetic diversity was determined by PCR ribotyping and enterobacterial repetitive intergenic consensus (ERIC) PCR. By these methods, 9 and 36 genotypes were found, respectively. The result showed that ERIC PCR analysis is a more discriminatory method than PCR ribotyping analysis and traditional serotyping scheme. We suggest that maximum discrimination can be achievied by a combination of these methods.

Wydawca

-

Rocznik

Tom

57

Numer

2

Opis fizyczny

p.157-163,fig.,ref.

Twórcy

autor
  • University of Podlasie, Prusa 12, 08-110 Siedlce, Poland
autor

Bibliografia

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  • Botes J., G. Willamson, V. Sinickas and V. Gurtler. 2003. Genomic typing of Pseudomonas aeruginosa isolates by comparison of riboprinting and PFGE: correlation of experimental results with those predicted from the complete genome sequence of isolate PAO1. J. Microbiol. Meth. 55: 231-240.
  • Czekajło-Kołodziej U., S. Giedrys-Kalemba and D. Mędrala. 2006. Phenotypic and genotypie characteristics of Pseudomonas aeruginosa strains isolated from hospitals in the north-west region of Poland. Pol. J. Microbiol. 55: 103-112.
  • Dawson S.L., J.C. Fry and B.N. Dancer. 2002. A comparative evaluation of five typing techniques for determining the diversity of fluorescent pseudomonads. J. Microbiol. Meth. 50: 9-22.
  • Dąbrowski W, U. Czekajło-Kołodziej, D. Mędrala and S. Giedrys-Kalemba. 2003. Optimization of AP-PCR fingerprinting discriminatory power for clinical isolates of Pseudomonas aeruginosa. FEMS Microbiol. Lett. 218: 51-57.
  • Ferrus M.A., M. Hernandez and J. Hernandez Haba. 1998. Ribotyping of Pseudomonas aeruginosa from infected patients: evidence of common strain types. APMIS 106: 456-462.
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  • Kingsford N.M. and H.W. Raadsma. 1995. Detection of Pseudomonas aeruginosa from ovine fleece washings by PCR amplication of 16S ribosomal RNA. Vet. Microbiol. Al: 61-70.
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Typ dokumentu

Bibliografia

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