The alpha2-adrenoceptors do not modify the activity of tyrosine hydroxylase, corticoliberine, and neuropeptide Y producing hypothalamic magnocellular neurons in the Long Evans and Brattleboro rats

• Autorzy: Bundzikova J. , Pirnik Z. , Zelena D. , Mikkelsen J.D. , Kiss A.
• Języki publikacji: EN

Abstrakty

EN

The hypothalamic supraoptic (SON) and paraventricular (PVN) nuclei are activated by body salt-fluid variations. Stimulation of 2-adrenoceptors by an agonist-xylazine (XYL) activates oxytocinergic but not vasopressinergic magnocellular neurons. In this study, tyrosine hydroxylase (TH), corticoliberine (CRH), and neuropeptide Y (NPY) magnocellular phenotypes, were analysed in response to 2-adrenoceptor manipulations and sustained hyperosmolality in vasopressin deficient homozygous Brattleboro (di/di) rats. Saline (0.9% NaCl, 0.1 ml/100g/bw), XYL (10 mg/kg/bw), atipamezole (ATIP, 2-adrenoceptors antagonist, 1 mg/kg/bw), and ATIP 5 min later followed by XYL, were applied intraperitoneally. Presence of immunolabeled Fos peptide signalized the neuronal activity. Ninety minutes after injections, the rats were anesthesized and sacrificed by transcardial perfusion with fixative. Coronal sections of 30 µm thickness double immunolabeled with Fos/neuropeptide were evaluated under light microscope. Under basal conditions, di/di in comparison with control Long Evans rats, displayed significantly higher number of TH, CRH, and NPY immunoreactive neurons in the SON and PVN (except NPY cells in PVN) and more than 90%, 75%, and 86% of TH, NPY, and CRH neurons, respectively, displayed also Fos signal in the SON. XYL did not further increase the number of Fos in the PVN and SON and ATIP failed to reduce the stimulatory effect of hypertonic saline in all neuronal phenotypes studied. Our data indicate that hyperosmotic conditions significantly influence the activity of TH, CRH, and NPY magnocellular neuronal phenotypes, but 2-adrenoceptors do not play substantial role in their regulation during osmotic challenge induced by AVP deficiency.

Słowa kluczowe

EN

alpha2-adrenoceptor; tyrosine hydroxylase; corticoliberine; neuropeptide Y; magnocellular neuron; rat; hypothalamic supraoptic; paraventricular nucleus; supraoptic nucleus; Fos-immunohistochemistry

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2010
• Tom: 61
• Numer: 4
• Opis fizyczny: p.391-398,fig.,ref.

Twórcy

autor

Bundzikova J.

• Slovak Academy of Sciences, 3 Vlarska Street, 833 06 Bratislava, Slovakia

autor

Pirnik Z.

autor

Zelena D.

autor

Mikkelsen J.D.

autor

Kiss A.

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