Ocena dzialania peptydow antydrobnoustrojowych z hodowli keratynocytow HaCaT na wybrane szczepy bakterii pochodzace ze zmian skornych

• Autorzy: Wiechula B. , Wilk I. , Ekiel A. , Tustanowski J. , Martirosian G.

Warianty tytułu

EN

The influence of AMPs from HaCaT keratinocytes on some bacteria strains derived from skin changes

• Języki publikacji: PL

Abstrakty

PL

Celem badania była ocena działania antydrobnoustrojowego białek pochodzących z ciągłej linii keratynocytów HaCaT na kliniczne izolaty szczepów bakterii Gram-dodatnich. Zbadano wpływ otrzymanego białkowego ekstraktu komórkowego (BEK) na wyżej wymienione szczepy. Zaobserwowano znaczne zróżnicowanie aktywności ekstraktu ze względu na czas inkubacji oraz rodzaj szczepu poddanego działaniu BEK.

EN

The aim of this study was to evaluate antimicrobial activity of protein extracts from HaCaT cell line against bacterial strains, isolated from clinical materials, obtained from patients with clinical symptoms of acne (Propionibacterium acnes) and gas gangrene (Clostridium perfringens and Sterptococcus pyogenes). Reference strain of Staphylococcus aureus ATCC 25923 also was used. Protein extracts from cultured HaCaT cells were obtained by 3-fold freezing/defreezing cells in dry ice following by centrifugation and incubated with appropriate bacterial suspension (0,5 McFarland scale) during 6 and 24 hours. We observed time-depending and strain-depending activity of HaCaT - protein extract. Interestingly, high activity was demonstrated against strains of S. pyogenes and C. perfringens. Because of increasing bacterial resistance to antibiotics further studies in the field of antimicrobial peptides are required.

Słowa kluczowe

PL

choroby czlowieka; choroby skory; zmiany skorne; bakterie; keratynocyty; peptydy antybakteryjne; ekstrakty peptydowe

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2006
• Tom: 58
• Numer: 2
• Opis fizyczny: s.163-168,tab.,bibliogr.

Twórcy

autor

Wiechula B.

• Slaska Akademia Medyczna w Katowicach, ul.Medykow 18, 40-752 Katowice

autor

Wilk I.

autor

Ekiel A.

autor

Tustanowski J.

autor

Martirosian G.

Bibliografia

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