Czestosc wystepowania genu AAC[6']-APH[2'']Ia wsrod gronkowcow i enterokokow wysokoopornych na antybiotyki aminoglikozydowe

• Autorzy: Jarzembowski T. , Michnowska-Swincow E. , Wisniewska K.
• Języki publikacji: PL

Abstrakty

PL

Celem badań było ustalenie czy wysoka oporność na aminoglikozydy wszystkich enterokoków i gronkowców izolowanych w regionie gdańskim uwarunkowane jest obecnością genu AAC(6')-APH(2")Ia. Wśród wszystkich objętych badaniami ziarenkowców stwierdzono wysoko- oporne szczepy nie posiadające tego genu.

EN

According to new reports the AAC (6')-APH (2")Ia gene is no longer the only gene encoding resistance to gentamycin in Gram positive cocci and therefore the current method for predicting synergism aminoglycosides wit bacterial cell wall active agents in this bacteria may need revision. To further our knowledge of aminoglycoside resistance mechanism in Gram positive cocci in Gdańsk region we tested presence of AAC (6')-APH (2")Ia gene among 22 enterococcal (E. faecalis) and 41 staphylococcal (S. haemoliticus, S. aureus, S. epidermidis) gentamycin resistant isolates. Presence of AAC (6')-APH (2")Ia gene varied from 50% (n=6) in gentamycin resistant S. epidermidis, 80% (n=10) in gentamycin resistant S. haemolyticus 88% in methicylin resistant Staphylococcus aureus (MRSA) (n=25). In Enterococcus faecalis this gene was noticed only in 59 % (n=22) of gentamycin resistant isolates. These results suggest that spread of resistance gene among different species is limited and AAC (6')-APH (2")Ia mediated gentamycin resistance mechanism is more common among MRSA and Staphylococcus haemoliticus.

Słowa kluczowe

PL

gen ACC; antybiotyki aminoglikozydowe; gronkowce; szczepy bakteryjne; opornosc na antybiotyki; bakterie

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2003
• Tom: 55
• Numer: 4
• Opis fizyczny: s.309-313,tab.,bibliogr.

Twórcy

autor

Jarzembowski T.

• Akademia Medyczna, Gdansk

autor

Michnowska-Swincow E.

autor

Wisniewska K.

Bibliografia

• 1. Busch-Sorensen C, Frimodt-Moller N, Miller G,H. Espersen F. Aminoglycoside resistance among Danish blood culture isolates of coagulase-negative staphylococci. APMiS 1996; 3: 873-80.
• 2. Chow JW. Aminoglycoside Resistance in Enterococci. Clin Infect Dis; 2000; 2; 586-9.
• 3. Chow JW, Zervos MJ, Lener SA. A novel gentamycin resistance gene in Enterococcus. Antimicrob Agents Chemother 1997; 41: 511^1.
• 4. Courvalin P. Interpretative reading of in vitro antibiotic susceptibility tests (the antibiogram). Clin Microbiol Infect 1996; 1: 26-34.
• 5. Dickgiesser N, Kreiswirth BN. Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridisation. Antimicrob Agents Chemother 1986; 45: 2930-32.
• 6. Dumssr H, Derlot E, Carlier C, Courvalin P. Gene homogeneity for aminoglycoside-modifying enzymes in gram-positive cocci. Antimicrob Agents Chemother 1990; 42: 164-8.
• 7. Gray G,S, Hnang T, Davies DJ. Aminocyclitol resistance in Staphylococcus aureus. Presence of plasmids and aminocyclitol-modifying enzymes. Plasmid 1983; 9: 147-58.
• 8. Huovinen P, Herva E, Katila ML i inni. Reliability of a disk diffusion method using semi-confluent growth in the determination of aminoglycoside resistance. Acta Path Microbiol Scand; 1986; 94: 153-7.
• 9. Kao SJ, You I, Clewell DB. Detection of the high-level aminoglycoside resistance gene aph(2")-Ib in Enterococcus faecium. Antimicrob Agents Chemother 2000; 44: 2876-9.
• 10. Kayser FH, Homberger F, Devaud M. Aminocyclitol modifying enzymes specified by chromosomal genes in Staphylococcus aureus. Antimicrob Agents Chemother 1978; 19: 766-72
• 11. Licitra CM, Brooks RG, Terry P i inni. Use of plasmid analysis and determinations of aminoglycoside modifying enzymes to characterise isolates from an outbreak of methicillin-resistant Staphylococcus aureus. J Clin Microbiol 1998; 27: 2535-8.
• 12. Lyon BR, Skurray R. Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiol Rev 1987; 16: 88-134.
• 13. Miller GH, Sabatelli F J, Naples L i inni. The most frequently occurring aminoglycoside resistance mechanism-combined result of surveys in eight regions of the world. J Chemother 1995; 5: 17-30.
• 14. Shaw KJ, Hare RS, Sabatelli FJ i inni. Correlation between aminoglycoside resistance profiles and DNA hybridisation of clinical isolates. Antimicrob Agents Chemother 1991; 35: 2253-61.
• 15. Shaw KJ, Rather PN, Hare RS, Miller GH. Molecular genetics of aminoglycoside resistance genes and familial relationship of the aminoglycoside-modifying enzymes. Microbiol Rev 1993; 5: 138-63.
• 16. Tsai SF, Zervos MJ, Clewell DB i inni. A nowel high-level gentamycin resistance gene, aph(2")-Id in Enterococcus sp. Antimicrob Agents Chemother 1998; 43: 1229-32.
• 17. Udo E, Dashti A. Detection of genes encoding aminoglycoside-modifying enzymes in staphylococci by polymerase chain reaction and dot blot hybridisation. International J Antimicrob Agents; 2000; 13: 273-9.
• 18. Vanhoof R, Godard C, Content J i inni. Detection by polymerase chain reaction of genes encoding aminoglycoside-modifying enzymes in methicillin-resistant Staphylococcus aureus isolates of epidemic phage types. J Med Microbiol 1994; 41: 282-90.
• 19. Wiśniewska K, Piechowicz L, Galiński J. Dominacja wieloopornych szczepów o zmniejszonej wrażliwości na kwas fusydowy wśród metycylinoopornych gronkowców złocistych izolowanych w regionie gdańskim. Polski Merkuriusz Lekarski 2000; 9; 746-9