Restriction polymorphisms of vitamin D receptor gene [VDR] - application of molecular genetic methods to recognise susceptible population to osteopenia nad osteoporosis

• Autorzy: Seremak-Mrozikiewicz A , Drews K. , Pienkowski W. , Bartkowiak-Wieczorek J. , Kurzawinska G. , Kopyra P. , Mrozikiewicz P.M.
• Języki publikacji: EN

Abstrakty

EN

Recently the significance of genetic traits, influencing hormonal and environmental factors, in susceptibility to osteopenia and osteoporosis development has been indicated. Much attention to the polymorphic variants of vitamin D receptor (VDR) gene was paid. The restriction polymorphisms in VDR gene could be involved in the modulation of vitamin D action. The VDR polymorphism influence level of bone mineral density (BMD) and cause the osteopenia and osteoporosis.124 postmenopausal women with osteopenia have been analysed (mean age 58.4±5.9 years). For detection of Taq\ VDR polymorphism PCR/RFLP (polymerase chain reaction/restriction fragment length polymorphism) assay have been used.The higher prevalence of 77" genotype (44.4?ó) in investigated group of postmenopausal women has been determined. The mean weight, height and BMI were the lowest in the TT group and the shortest time since last menopause in this group has been observed. In TT group the lowest BMD level and f-score value was determined (0.939 g/cm- and -2.18, respectively). The same observation we have found if comparing the young adults (YA) (78.3°o) and age matched (AM) (88.1°o) index (Tab. 1). The slight tendency to lower age of last menopause in the group of women with genotype containing at least one Tállele (TT + Tí) 49.5±4.2 vs. 50.9±3.8 years has been detected. The presence of Tállele of VDR polymorphism could predict the lower bone mineral density and ¡-score value and connect with early occurrence of last menses in the group of post­menopausal women. For founding a clearly correlation between TaqI polymorphism and appearance of osteopenia and osteoporosis further investigations are necessary.

PL

W ostatnich latach wskazano na wieloczynnikowe podtoże osteopenii i osteoporozy, za­leżne od wpływów hormonalnych, środowiskowych i genetycznych. Dużo uwagi poświęca się polimorficznym wariantom genu receptora witaminy D (VDR), które mogą być włączone w mo­dulację odpowiedzi na jej działanie. Sugeruje się również, że polimorfizm VDR wpływa na waitość gęstości mineralnej kości (BMD) oraz jest przyczyną rozwoju osteopenii i osteoporozy. W pracy badano grupę 124 kobiet po menopauzie z osteopenią (średni wiek 58,4±5,9 lat). Polimorfizm TaqI genu VDR analizowano z zastosowaniem reakcji łańcuchowej po- limerazy oraz metody długości fragmentów restrykcyjnych (PCR/RF1.P assays). Obserwowano przewagę genotypu TT (44,4%) w grupie badanej kobiet po menopauzie. W grupie TT odnotowano najniższą średnią masę ciała, wzrost, wskaźnik RMI oraz najkrótszy śred­ni czas od wystąpienia ostatniej miesiączki. W grupie tej wyznaczono również najniższą wartość wskaźnika

Słowa kluczowe

EN

molecular genetic method; vitamin D receptor gene; recognition; application; restriction polymorphism; osteopenia; osteoporosis; susceptible population

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2004
• Tom: 50
• Numer: 3-4
• Opis fizyczny: p.168-174,fig.,ref.

Twórcy

autor

Seremak-Mrozikiewicz A

• University of Medical Sciences, Poznan, Poland

autor

Drews K.

autor

Pienkowski W.

autor

Bartkowiak-Wieczorek J.

autor

Kurzawinska G.

autor

Kopyra P.

autor

Mrozikiewicz P.M.

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