Allelic variation of the porcine alpha-1,3-galactosyltransferase 1 [GGTA1] gene

• Autorzy: Day A , Rocha D
• Języki publikacji: EN

Abstrakty

EN

The alpha- 1,3-galactosyltransferase 1 enzyme (GGTA1) produces the α-Gal epitopes, responsible for pig-to-human hyperacute xenograft rejection. Recently, efforts have been directed at inactivating the porcine GGTA1 gene in order to reduce hyperacute rejection. As very little is known about the genetic variability of this key gene among pig breeds, we investigated the variation in its nucleotide sequence, by amplification of the entire coding region with the use of polymerase chain reaction followed by DNA sequencing. Eight commercial pig populations were analysed and 17 single nucleotide polymorphisms (SNPs) were detected: 11 in intronic regions and 6 in the 3' untranslated region (UTR). No SNPs change the encoded protein; however, 8 of these SNPs may alter the transcriptional regulation and pre-mRNA splicing of GGTA1.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2008
• Tom: 49
• Numer: 1
• Opis fizyczny: p.75-79,ref.

Twórcy

autor

Day A

• Genus pic, Genus Cambridge Research Laboratory, University of Cambridge, Cambridge, U.K.
• Current address: INRA - Université de Limoges, Limoges, France

autor

Rocha D

• Current address: INRA - Université de Limoges, Limoges, France

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