Koniugacyjne przekazywanie genow opornosci na glikopeptydy i makrolidy w obrebie rodzaju Enterococcus oraz ze szczepow Enterococcus faecalis do Staphylococcus aureus

• Autorzy: Mlynarczyk A. , Mlynarczyk G. , Luczak M.
• Języki publikacji: PL

Abstrakty

PL

Oceniano możliwość koniugacyjnego przekazywania plazmidów warun#kujących oporność na makrolidy oraz oporność typu VanA na antybiotyki glikopeptydowe z klinicznych izolatów E. faecalis do E. faecalis ATCC 29212 oraz do metycylino-opornych i metycylino-wrażliwych szczepów Staphylococcus aureus.

EN

The resistance determinants were transferred from clinical strains of enterococci to Staphylococcus aureus strains. As recipients methicillin - resistant and methicillin- susceptible strains were used and the filter - mating procedure was performed. The transconjugants resistant to erythromycin were obtained in the case of all recipients. in one case the vanA determinant conferring the resistance to vancomycin was transferred together with erythromycin resistance. However the resistance was very unstable and the level was not as high as in the case of Enterococcus faecalis donor strain. The vanA determinant was easily transferred between enterococcal strains by the conjugation and a transfer occurred of vanA alone or together with erythromycin resistance.

Słowa kluczowe

PL

Enterococcus faecalis; plazmidy; koniugacja; szczepy bakteryjne; opornosc na antybiotyki; mikrobiologia lekarska; transdukcja; geny odpornosci; opornosc na glikopeptydy; bakterie; opornosc na makrolidy; Staphylococcus aureus

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2002
• Tom: 54
• Numer: 1
• Opis fizyczny: s.21-28,fot.,rys.,bibliogr.

Twórcy

autor

Mlynarczyk A.

• Akademia Medyczna, Warszawa

autor

Mlynarczyk G.

autor

Luczak M.

Bibliografia

• 1. Biavasco F, Giovanetti E, Miele A i inni. In vitro conjugative transfer of VanA vancomycin resistance between Enterococci and Listeriae of different species. Eur J Clin Microbiol Infect Dis 1996; 15: 50-59.
• 2. Carias LL, Rudin SD, Donskey CJ, Rice LB. Genetic linkage and cotransfer of a novel, vanB-containing transposon (Tn5382) and a low-affinity penicillin-binding protein 5 gene in a clinical vancomycin-resistant Enterococcus faecium isolate. J Bacteriol. 1998; 180: 4426-34.
• 3. Clewell DB, An FY, White BA, Gawron-Burke C. Sex pheromones and plasmid transfer in Streptococcus faecalis: a pheromone cAM373, which is also excreted by Staphylococcus aureus. Basic Life Sci 1985; 30: 489-503.
• 4. Dutka-Malen S, Evers S, Courvalin P.: Detection of glicopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol 1995; 33: 24-27.
• 5. Heaton MP, Discotto LF, Pucci MJ, Handwerger S. Mobilization of vancomycin resistance by transposon-mediated fusion of a VanA plasmid with an Enterococcus faecium sex pheromone-response plasmid. Gene 1996; 171: 9-17.
• 6. Heaton MP, Handwerger S. Conjugative mobilization of a vancomycin resistance plasmid by a putative Enterococcus faecium sex pheromone response plasmid. Microb Drug Resist 1995; 1: 177-83.
• 7. Jeljaszewicz J, Młynarczyk G, Młynarczyk A. Present and future problems of resistance in Gram-positive cocci. Infection 1998; 26: 1-6.
• 8. Jeljaszewicz J, Młynarczyk G, Młynarczyk A. Antibiotic resistance in Gram- positive cocci. Int J Antimicrob Agents 2000; 16: 473-78.
• 9. Jones JM, Yost S.C, Patte PA. Transfer of the conjugal tetracycline resistance transposon Tn916 from Streptococcus faecalis to Staphylococcus aureus and identification of some insertion sites in the staphylococcal chromosome. J Bactcriol 1987; 169: 2121-31.
• 10. McAshan SK, Vergin KL, Glovannoni SJ, Thaler DS. Nucleotide interspecies recombination between enterococci: genetic and phenotypic diversity of vancomycin- resistant transconjugants. Microb Drug Resist 1999; 5: 101-12.
• 11. Młynarczyk A, Młynarczyk G, Jeljaszewicz J. The genome of Staphylococcus aureus: a review. Zbl Bakteriol 1998; 287: 277-314.
• 12. Noble WC, Virani Z, Cree RGA. Co-transfer of vancomycin and other resistance genes from Enterococcus faecalis NCTC 12201 to Staphylococcus aureus. FEMS Microbiol Lett 1992; 93: 195-98.
• 13. Noble WC, Rahman M, Karadec T, Schwarz S. Gentamicin resistance gene transfer from Enterococcus faecalis and E. faecium to Staphylococcus aureus, S. intermedius and S. hyicus. Vet Microbiol. 1996; 52: 143-52.
• 14. Petts DN, Noble WC, Howell SA. Typing methods for a study of gene transfer in enterococci. Adv Exp Med Biol 1997; 418: 413-15.
• 15. Quintiliani R Jr, Courvalin P. Conjugal transfer of the vancomycin resistance determinant vanB between enterococci involves the movement of large genctic elements from chromo#some to chromosome. FEMS Microbiol Lett 1994; 119: 359-63.
• 16. Rice LB. Tn916 family conjugative transposons and dissemination of antimicrobial resistance determinants. Antimicrob Agents Chemother 1998; 42: 1871-77.
• 17. Riedl S, Ohlsen K, Werner G i inni. Impact of flavophospholipol and vancomycin on conjugational transfer of vancomycin resistance plasmids. Antimicrob Agents Chemother 2000; 44: 3189-92.
• 18. Schaberg DR, Clewell DB, Glatzer L. Conjugative transfer of R-plasmid from Streptococcus faecalis to Staphylococcus aureus. Antimicrob Agents Chemother 1982; 22: 204-7.
• 19. Udo EE, Grubb WB. Conjugal transfer op plasmid pWBG637 from Staphylococcus aureus to Staphylococcus epidermidis and Streptococcus faecalis. FEMS Microbiol Lett 1990; 60; 183-87.
• 20. Woodford N, Morrison D, Johnson AP i inni.Plasmid-mediated vanB glycopeptide resistance in enterococci. Microb Drug Resist 1995; 1: 235-40.