The application of FPLC chromatography [Mono Q] for the purification of wheat RNA polymerases II and III

• Autorzy: Glanc P , Galbas M. , Dullin P. , Szalata M. , Slomski R.
• Języki publikacji: EN

Abstrakty

EN

Transcription is the main step in the regulation of gene expression. To study this process in vitro, it is necessary to obtain highly purified RNA polymerases. Here, we describe a method of RNA polymerase purification using a Mono Q FPLC column. Using Mono Q column chromatography accelerates the purification process and separates RNA polymerase II from RNA polymerase III with good yield.

Słowa kluczowe

EN

transcription; wheat; purification; RNA polymerase

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2003
• Tom: 08
• Numer: 3
• Opis fizyczny: p.825-830,fig.

Twórcy

autor

Glanc P

• Agricultural University, Wolynska 35, 60-637 Poznan, Poland

autor

Galbas M.

autor

Dullin P.

autor

Szalata M.

autor

Slomski R.

Bibliografia

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