Photodynamic effect of protoporphyrin diarginate [PPArg2] on methicillin-resistant Staphylococcus aureus and human dermal fibroblasts

• Autorzy: Grinholc M. , Kawiak A. , Kurlenda J. , Graczyk A. , Bielawski K.P.
• Języki publikacji: EN

Abstrakty

EN

The worldwide rise in the antibiotic resistance of bacteria forces the development of alternative antimicrobial treatments. A potential approach is photodynamic inactivation (PDI). The aim of the present study was to determine the phototoxicity of protoporphyrin diarginate (PPArg2) against methicillin-resistant Staphylococcus aureus and human dermal fibroblasts. Different concentrations (0 to 20 µM) of PPArg2 and light dose of 6 J cm -2 were tested. Cell viability was evaluated using the methylthiazoletetrazolium (MTT) assay. Incubation with 10 µM followed by illumination yielded a 3.6 log10-unit reduction in the viable count for Staphylococcus aureus. At the same experimental conditions, only 22.5% of the fibroblasts were photoinactivated. Protoporphyrin diarginate at concentrations up to 20 µM demonstrated no toxicity towards S. aureus or fibroblasts when not irradiated. These results suggest that the protoporphyrin diarginate exerts a high bactericidal effect against methicillin-resistant S. aureus strain without harming eukaryotic cells.

Słowa kluczowe

EN

antimicrobial photodynamic inactivation; dermal fibroblast; methicillin resistance; human disease; protoporphyrin diarginate; antibiotic resistance; bacterial strain; phototoxicity; cytotoxicity; Staphylococcus aureus

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2008
• Tom: 55
• Numer: 1
• Opis fizyczny: p.85-90,fig.,ref.

Twórcy

autor

Grinholc M.

• University of Gdansk, Gdansk, Poland

autor

Kawiak A.

autor

Kurlenda J.

autor

Graczyk A.

autor

Bielawski K.P.

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