Detection of Flavobacterium psychrophilum in fry of rainbow by RT-PCR

• Autorzy: Vardic I , Kapetanovic D. , Teskeredzic Z. , Teskeredzic E.
• Języki publikacji: EN

Abstrakty

EN

The paper describes the occurence of Flavobacterium psychrophilum as a concurrent pathogen with infectious hematopoietic necrosis virus in rainbow trout fry in Croatia. A molecular technique was applied for F. psychrophilum detection due to the failure of standard bacterial identification on agar plates. As the tissue material had already been used up for RNA extraction and detection of RNA virus, classical, well-described PCR could not be performed. Consequently, one step RT-PCR was adapted for F. psychrophilum identification. This method proved to be a valuable diagnostic tool for F. psychrophilum detection in mixed co-infections with RNA viruses.

Słowa kluczowe

EN

Croatia; fish farm; freshwater farm; rainbow trout; Oncorhynchus mykiss; Coho salmon; Oncorhynchus kisutch; mortality; economic loss; etiological agent; Flavobacterium psychrophilum; detection; infection; animal disease; anaemia; gill; internal organ; disease symptom; skin ulcer; fin necrosis; silver salmon zob.Coho salmon

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 09
• Opis fizyczny: p.1005-1006,fig.,ref.

Twórcy

autor

Vardic I

• Ruder Boskovic Institute, Bijenicka c.54, Zagreb 10 000, Croatia

autor

Kapetanovic D.

autor

Teskeredzic Z.

autor

Teskeredzic E.

Bibliografia

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