The role of plant growth regulators on direct and indirect plant regeneration from various organs of Leucaena leucocephala

• Autorzy: Maity S , Ray S. , Banerjee N.
• Języki publikacji: EN

Abstrakty

EN

Prolific differentiation of shoot buds of Leucaena leucocephala was induced from the different plant parts viz. cotyledon, hypocotyl and leaf. Adventitious shoot bud formation was recorded with prudent application of N6-2- (isopentenyl) adenosine and 15 % (v/v) coconut water. Coconut water alone was unable to produce any beneficial effect with regard to the shoot bud proliferation but the response was augmented with the increase in concentration of N6-2- (isopentenyl) adenosine. However supra-optimal level of N6-2- (isopentenyl) adenosine was inhibitory. Best response was recorded from the cotyledon explant at 2 mg·dm-3 N6-2- (isopentenyl) adenosine compared to the other two explants. Comparative assessment was undertaken following the same experimental protocol in liquid shake culture. The regenerated shoot buds were subcultured in plant growth regulator-free medium where leafy shoot emergence was recorded. Optimum regeneration of roots was observed in these shoots in presence of 1 mg dm-3 α-naphthalene acetic acid. Plantlets were finally hardened following standard procedures before transplantation to the field. In another experimental set up, the de-embryonated cotytedons regenerated shoot buds via callus formation. The regenerated shoots and plantlets obtained through callus mediated organogenesis could be used for rapid multiplication and also for the genetic improvement of individual clones of Leucaena leucocephala.

Słowa kluczowe

EN

plant growth regulator; plant regeneration; plant biotechnology; callus; growth regulator; organ; plant growth; Leucaena leucocephala

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2005
• Tom: 27
• Numer: 4A
• Opis fizyczny: p.473-480,fig.,ref.

Twórcy

autor

Maity S

• Visva-Bharati University, Santiniketan-731235, West Bengal, India

autor

Ray S.

autor

Banerjee N.

Bibliografia

• Arditti J., Ernst R. 1993. Micropropagation of orchids. John Wiley and Sons, NewYork :38-39
• Badere R.S., Koche D.K., Pawar S.E., Choudhary A.D. 2002. Regeneration through multiple shooting in Vigna radiata. Journal of Cytology and Genetics, 3: 185-190.
• Bonga J.M., Aderkas P. von, James D. 1987. Potential applicaiion of hapioid culiures of tree species. In: Genetic manipulation of woody plants ed. by J.W. Hanover and D.E. Keathley, Plenum Press, New York, 57-77.
• Dutta S.K., Dutta K. 1985. Auxin and KNO3 induced regeneration of Leguminous tree Leucaena leucocephala through tis iue culiure. Current Science, 54: 248-250.
• Flinn B.S., Webb D.T., Glorgis W. 1980. In vitro control of caulogenesis by growth reguiators and media components in embryonic explants of eastern white pine (Pinus strobes). Canadian Journal of Botany, 64: 1948-1956.
• Gharyal P.K., Maheswari S.C. 1990. Differentiation in explants from mature leguminous trees. Plant Cell Reports, 8: 550-553.
• Ghosh P.D., Bandyopadhyay S. 1984. Tissue Culture studies of leguminous trees with particular reference to Leucaena glauca. Applied Biotechnology of Medicinal and Aromatic Timber Plants, 1: 203-210.
• Goh H.K., Rao A.N., Loh C. 1990. Direct shoot bud formation from leaf explants of seedlings and mature mangosteen (Garcinia mangostana L.) trees. Plant Science, 68: 113-121.
• Hossain M., Hossain T., Hossain S.N. 1992. Morphogenic responses in explants of Leucaena leucocephala (Lam.) de wit following different phytohormones. Plant Cell Tissue and Organ Culture, 2: 31-34.
• Jain M., Chaturvedi H.C. 2004. In vitro cloning of Cajanus cajan var. Bahar through prolific shoot bud differentiation in leaf segments and production of fertile plants. Indian Journal of Biotechnology. 3: 258-262
• John A, Webb K.J. 1997. Sitka spruce (Picea sitchensis (Bong.) Carr.) In: Cell and Tissue Culture in Forestry, Case Histories: Gymnosperms, Angiosperms and Palms, ed. by J.M. Bonga, D.J. Durzan, Martinus Nijhoff Publishers, Dordrecht, 3: 30-41.
• Khattar S., Mohan Ram H.Y. 1982. In vitro morphogenesis in Sesbania sesban from different explants. Indian Journal of Experimental Biology, 20: 216-29.
• Little T.M., Hills F.J. 1978. Agricultural experimentation. John Wiley and Sons, New York: 45-46
• Mary Mathew K., Rao Y.S., Kumar P.K., Madhusudanan K.J., Potty S.N. 1999. In vitro culture system in Vanilla. In: Plant Tissue Culture and Biotechnology: Emerging Trends. ed. by P.B. Kavikishor, Universities Press, India :171-179.
• Mc Kently A.H., Moore G.A., Gardner F.P. 1990. In vitro plant regeneration of peanut from seed explants. Crop Sci., 30: 192-196.
• Mitra G.C., Chaturvedi, H.C. 1970. Fruiting plants from in vitro grown leaf tissue of Rauwolfia serpentina. Current Science, 39: 128-129.
• Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15: 473-499.
• Nagmani R. Venkateswaran S. 1979. In vitro culture of hypocotyl and cotyl edon segments of Leucaena. Leucaena Research Reports, 4: 88-89.
• Negi S.S., Singh B., Pal Singh M. 1995. In: Manual of Indian Forestry, ed. by Bawa, Khosla, Kohli, MPS Publication, India, Vol- 4: 145-147.
• Raju C.R., Kumar P.P., Chandramohan M., Iyer R.D. 1984. Direct organogenesis from leaf explants of Cocos nucifera. Journal of Plant Crops., 12: 75-76.
• Ravishankar R.V., Jagadishchandra K.S. 1989. Micropropagation of Indian Rosewood by tissue culture. Annals of Botany, 64: 43-46.
• Romberger J.A., Tabor C. 1971. The Picea abies shoot apical meristem in culture. 1. agar and autoclaving effects. American Journal of Botany, 58: 131-140.
• Roy J., Banerjee N. 2000. Rhizome and shoot development during in vitro propagation of Geodorum densiflorum (Lam.) S Chltr. Scientia Horticulturae, 94: 181-192.
• Singh R.R., Bisht R.P., Toky O.P. 1995. Problems of seed collection and technology in the exploitation of forest resources. In : Manual of Indian Forestry, ed. by Bawa, Khosla and Kohli), MPS Publication, India, 4: 87-91.
• Skoog F. Miller C.O. 1957. Chemical regulation of growth and organ formation in plant tissues cultivated in vitro In: Biological Action of Growth Substances. 11th Symposium of Society of Experimental Biology, 11: 118: 131.
• Sreedevi E., Pullaiah T. 1999. In vitro plantlet regeneration from seedling explants of Dalbergia paniculata Roxb. In: Plant Tissue Culture and Biotechnology, Emerging trends. ed. By P.B. Kavikishor, Universities Press, India: 112-117.
• Suttle G.R.L. 1996. Commercial laboratory production. In: Plant tissue culture concepts and laboratory exercises. ed. by R.N. Trigiano, D.J. Gray, CRC Press, Inc., New York: 331-339.
• Venkateswaran S., Gandhi V. 1982. Mass propagation and genetic improvement of forest trees by biomass production by tissue culture. Biomass, 2: 5-15.
• Yasodha R., Sumathi R., Gurumurthi K. 2004. Micropropagation for quality propagule production in plantation forestry. Indian Journal of Biotechnology, 3 : 159-170.