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1998 | 44 | 1 |

Tytuł artykułu

Histochemiczne badania aktywnosci dehydrogenazy mleczanowej w ukladzie Fasciola hepatica-Mus musculus

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Warianty tytułu

Języki publikacji

PL

Abstrakty

EN
A histochemical assay was elaborated to study lactate dehydrogenase activity in the tissues of Fasciola hepatica and in the liver of mice during the course of fasciolosis. LDH activity was determined in the tegument, muscles, parenchyma, oral sucker and the caecum of juvenile and adult liver flukes inhabiting the host liver parenchyma and bile ducts. The most substantial changes in LDH activity were recorded in the tegument outer surface layer. White mice of CFW strain were infected with 10, 30 and 50 doses of F. hepatica metacerkariae administered per os. A dramatic decrease in LDH activity in the experimental mice liver during the course of infection, particularly in the acute fasciolosis was observed.

Wydawca

-

Rocznik

Tom

44

Numer

1

Opis fizyczny

s.53-62, rys.,tab.,fot.,bibliogr.

Twórcy

  • Akademia Medyczna, Powstancow Wielkopolskich 72, 70-111 Szczecin

Bibliografia

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  • BUNDENSEN P. G., JANSSENS P. A. 1971. Biochemical tracing of parasite infections. I. Fasciola hepatica L. in mice - a qualitative study. Int. J. Parasitol. 1: 7-14.
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  • DOW C., ROSS J. G., TODD I. R. 1967. The pathology of experimental fascioliasis in calves. J. Camp. Pathol. 77: 377-385.
  • - - - 1968. The histopathology of Fasciola hepatica infections in sheep. Parasitology 58: 129-135.
  • FACINO M. R., CARINl M. GENCHI C., TOFANETII O., CASCIARRI I. 1989. Participation of lipid peroxidation in the loss of hepatic drug-metabolizing activities in experimental fascioliasis in the rat. Pharmacol. Res. 21: 549-560.
  • - - - - - BEDOSCHI D. 1990. Antihepatoxic properties of uridine-diphosphoglucose in liver fluke infection. Arzeneim. Forsch./ Drug. Res. 40: 490-498.
  • GALTIER P., CAMBON-GRAS C., FERNANDEZY., DELTOUR P., EECKHOUTTE C., HOELLINGER H. 1994. Fasciola hepatica: liver microsomal membrane functions in host rat. Exp. Parasitol. 78: 175-182.
  • HANISH M. J. E., ToPFER F., LENTON L. M., BEHM C. A., BYGRAVE F. L. 1992. Restoration of mitochondrial energy-linked reactions following dexamethasone treatment of rats infected with the liver fluke Fasciola hepatica: Biochim. Biophys. Acta 1139: 196-202.
  • KOŁODZIEJCZYK L. 1994. Study on glycogen con tent in the liver of mouse vs. pathomorphological changes in experimental fasciolosis. Acta Parasitol. 39: 211-216.
  • - 1996a. Histochemical studies on Fasciola hepatica in mice. I. Oxidoreductase activity in juvenile and adult liver flukes. Ibid. 41: 214-220.
  • - 1996b. Histochemical studies on Fasciola hepatica in mice. II. Oxidoreductase activity in experimentally induced fasciolosis. Ibid. 41: 221-226.
  • LENTON L. M., BEHM C. A., BYGRAVE F. L. 1994. Characterization of the oligomycine-sensitivity properties of the F1F0= ATPase in mitochondria from rats infected with the liver fluke Fasciola hepatica. Biochim. Biophys. Acta 1186: 237-242.
  • - - - 1995. Aberrant mitochondrial respiration in the livers of rats infected with Fasciola hepatica: the role of elevated non-esterified fatty acids and altered phospholipid composition. Biochem. J. 307: 425-431.
  • LLOYD G. M. 1986. Energy metabolism and its regulation in the adult liver fluke Fasciola hepatica. Parasitology 93: 217-248.
  • MARTINEZ-MORENO A., MARTINEZ-MORENO F. J., JIMENEZ V., ACOSTA I. 1996. Pathological, pathophysiological and parasitological study on the susceptibility of goat to primary and secondary Fasciola hepatica infections. Parassitologia 38, EMOP VII Parma, 2-6 September 1996, Abstracts: 431.
  • MURRAY R. K., GRANNER D. K., MAYES P. A., RODWELL V. W. 1995. Biochemia Horpera (tł. F. Kokot). Wydawnictwo Lekarskie PZWL, Warszawa.
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  • PRICHARD R. K., SCHOFIELD P. J. 1968a. The metabolism of phosphoenolpyruvate and pyruvate in the adult liver fluke. Fasciola hepatica. Biochim. Biophys. Acta. 170: 63-76.
  • - - 1968b. The glycolytic pathway in the adult liver fluke, Fasciola hepatica. Camp. Biochem. Physiol. 24: 697-710.
  • RULE C. J., BEHM C. A., BYGRAVE F. L. 1989. Aberrant energy-linked reactions in mitochondria isolated from the livers of rats infected with the liver fluke Fasciola hepatica. Biochem. J. 260: 517-523.
  • RUSHTON B., MURRAY M. 1977. Hepatic pathology of a primary experimental infection of Fasciola hepatica in sheep. J. Camp. Pathol. 87: 459-470.
  • STURM G., HIRSCHÄUSER C., ZILLIKEN F. 1969. Vergleichende Bestimmung von Enzymaktivitäten in Fasciola hepatica und Rindleber. Hoppe-Seyler's Z. Physiol. Chem. 30: 696-700.
  • TAYLOR E. L., MOZLEY A. 1948. A culture method for Lymnaea truncatula. Nature 161: 894.
  • THORPE E. 1965. The pathology of experimental fascioliasis in the albino rat. J. Camp. Pathol. 75: 39-44.
  • THREADGOLD L. T. 1985. Fasciola hepatica: interaction of tegument with poly-L-lysine and enzymes. Exp. Parasitol. 59: 220-230.
  • TIELENS A. G. M. 1994. Energy generation in parastic helminths. Parasitol. Today 10: 346-352.
  • - VAN DEN HEUVEL J., VAN DEN BERGH S. G. 1984. The energy metabolism of Fasciola hepatica during its development in the final host. Mol. Biochem. Parasitol. 13: 301-307.
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  • TSOCHEVA-GAITANJIEVA N. T., WESSELINOVA D., RUPOVA-POPJORDANOVA L., TOSHKOVA R. 1996. Biologically active substances of host and parasite origin under fascioliasis. Parassitologia 38, EMOP VII, Parma, 2-6 September 1996, Abstracts: 152.
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Bibliografia

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