Self-incompatibility alleles in Polish wild pear [Pyrus pyraster [L.] Burgsd.]: a preliminary analysis

• Autorzy: Wolko L , Antkowiak W , Sips M , Slomski R
• Języki publikacji: EN

Abstrakty

EN

Wild pear (Pyrus pyraster, syn. P. communis var. pyraster) is thought to be one of the species that gave rise to all other members of the genus Pyrus, although intraspecific hybridizations with cultivated varieties could cause the disappearance of original species characteristics. S-RNase alleles from 7 different wild pear individuals, collected from various regions of Poland, were cloned on the basis of the PCR method and nucleotide sequence analyses. The hypervariable (HV) region is responsible for allele-specific S-RNase activity in the self-incompatibility mechanism. The high level of polymorphism of its sequences may constitute a source of valuable phylogenetic information. From all individuals, 14 sequences were obtained successfully, and 9 of them were novel alleles. Phylogenetic analysis of these alleles was based on the amino acid sequence interpretation of coding regions and intron nucleotide sequences. The research conducted on a limited pool of available P. pyraster alleles gives only an initial insight into possible S-RNase allele polymorphisms in wild populations. At this stage, the results do not confirm a strong influence of cultivated pear species on the wild pear.

Słowa kluczowe

PL

pear; wild pear; Polish wild pear; Pyrus pyraster; polymerase chain reaction; allele; self-incompatibility; S-RNase; cultivated species; wild plant; genetic variability

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2010
• Tom: 51
• Numer: 1
• Opis fizyczny: p.33-35,ref.

Twórcy

autor

Wolko L

• Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Wolynska 35, 60-637 Poznan, Poland

autor

Antkowiak W

• Department of Botany, Poznań University of Life Sciences, Poznań, Poland

autor

Sips M

• Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Wolynska 35, 60-637 Poznan, Poland

autor

Slomski R

• Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Wolynska 35, 60-637 Poznan, Poland

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