Efekt terapeutyczny niektorych antybiotykow w doswiadczalnych zakazeniach gronkowcowych oraz jego powiazania z aktywnoscia in vitro antybiotykow w stezeniach subinhibicyjnych wobec szczepow Staphylococcus aureus

• Autorzy: Kaczmarska L. , Jakoniuk P.
• Języki publikacji: PL

Abstrakty

PL

W badaniach in vivo użyto kloksacyliny, gentamycyny i linkomycyny oraz szczepów Staphylococcus aureus o zbliżonych wartościach MIC, ale odmiennej podatności na subinhibicyjne stężenia antybiotyków (sub-MIC). Wykazano, że dawki efektywne ED 50 tych antybiotyków były istotnie niższe u myszy Swiss albino zakażonych szczepami S. aureus podatnymi na sub - MIC. Myszy z takimi zakażeniami były również skutecznie leczone tymi antybiotykami.

EN

The aim of the study was to demonstrate of whether the therapeutic effects of antibiotics depend on their in vitro activity in sub-inhibitory concentrations against staphylococci. Cloxacillin, gentamicin and lincomycin were used in the study. Groups of S. aureus strains, containing 6 strains with similar MIC values each but different sensitivity to sub-inhibitory antibiotic concentrations (sub-MIC) were selected (a total of 36 trains): i. strains increasing their sensitivity to phagocytosis and bactericidal activity of rabbit leukocytes after incubation with an antibiotic in 0,1 MIC concentration, ii. strains with sensitivity to the above factors unaffected by incubation with an antibiotic in 0.5 MIC concentration. The doses of staphylococci causing death of 90 - 100% of Swiss albino mice 10 days after і p. infection were determined. The injected doses (LD 90-100) and various doses of antibiotics were used to determine ED50 values as well as the survival rate of the mice with experimental staphylococcal infections after treatment with these antibiotics. It was demonstrated that effective doses (ED 50) of the antibiotics were significantly lower when the antibiotics were administered once to mice infected with strains S. aureus sensitive to sub-MIC concentrations of the investigated antibiotics than for mice infected with strains resistant to their sub-MIC concentrations. Similar correlations were observed in mice which were given the antibiotics several times (for 7 days): the percentage of the surviving mice was higher in the group infected with sub-MIC sensitive strains. The therapeutic effect of cloxacillin, gentamicin and lincomycin demonstrated a significant correlation with the S. aureus strains used to induce the infections and their sensitivity, or lack of sensitivity in vitro, to phagocytosis and bactericidal activity of leukocytes in the presence of antibiotics in sub-MIC concentrations.

Słowa kluczowe

PL

infekcja bakteryjna; kloksacylina; antybiotyki; gentamycyna; leczenie; efekt terapeutyczny; linkomycyna; Staphylococcus aureus

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2003
• Tom: 55
• Numer: 1
• Opis fizyczny: s.1-10,tab.,rys.,bibliogr.

Twórcy

autor

Kaczmarska L.

• Akademia Medyczna, Bialystok

autor

Jakoniuk P.

Bibliografia

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