Genetic distances in hens estimated with protein genes frequencies and procedures of DNA analysis

• Autorzy: Brodacki A. , Tarkowski J. , Flis J.
• Języki publikacji: EN

Abstrakty

EN

Egg yolk and white proteins from hens kept in eight conservation flocks in Poland were separated with horizontal polyacrylamide gel electrophoresis. The analyses were done on 100-120-egg samples from each of the following breeds: Green-Legged Partridge, ZKF and ZK11, Yellow-Legged Partridge, Z33, Polbar, PB, Leghorn, H22 and G99, Rhode Island Red, RD2, and Sussex, S66. After estimating phenotype frequency based on 30-35 bands on the electrophoregrams, the following were identified: gene frequencies of fast-migrating prealbumins, Pa-F, and transferrins, TF, in egg yolk, as well as ovalbumins Ov-A, ovoglobulins, G3, G4, G2, and conalbumins, Co, in the white of egg. Similarity and Nei's genetic distances were calcula-ted according to gene frequency (GF) and procedures used in analysing DNA polymorphism, based on frequencies of particular protein bands (BF) and band sharing (BS). Application of GF and BF in the evaluation of genetic distances gave similar results.

Słowa kluczowe

EN

protein gene; egg yolk; DNA analysis; hen; protein polymorphism; animal production; genetic distance; procedure; white protein; chicken

• Wydawca: -
• Czasopismo: Electronic Journal of Polish Agricultural Universities. Series Animal Husbandry
• Rocznik: 2003
• Tom: 06
• Numer: 2
• Opis fizyczny: http://www.ejpau.media.pl/series/volume6/issue2/animal/art-08.html

Twórcy

autor

Brodacki A.

• Agricultural University of Lublin, Akademicka 13, 20-033 Lublin, Poland

autor

Tarkowski J.

autor

Flis J.

Bibliografia

• Bjornstadt G., Roed K. H., 2001. Breed demarcation and potential for breed allocation of horses assessed by microsatellite markers. Anim Gen. 32, 59-65.
• Brodacki A., Zieba G., Cywa-Benko K., 2001. Genetic distance between selected breeds and lines of laying hens. Electron. J. Pol. Agric. Univ., Animal Husbadry 4, 2, 2001.
• Bumstead N., Messer L. I., Greenwood N. G., 1987. Use of ev loci as a measure of inbreeding in domestic fowls. Brit. Poult. Sci. 28, 717-725.
• Deepak G. Y., Kumar S., Singh R. K., Butchaiah G., Johari D. C., 1998. DNA polymorphism in various White Leghorns lines by RAPD-PCR. Proc. 10th European Poult. Conf. Jerusalem, Israel 21-26 June 1998. wPSA – Israel Branch, 230-233.
• Gahne B., Juneja R. K., Grolmus J., 1977. Horizontal polyacrylamide gradient gel electrop-horesis for simultaneous phenotyping of transferrin, post-transferrin, albumin and postalbumin in the blood plasma of cattle. Anim. Blood Groups Biochem. Genet. 8, 127-137.
• Hashiguchi T., Tsuneyoshi M., Nishida T., Higashiwutoko H., Hiraoka E., 1981. Phylogenetic relationship determined by the blood proteins types of fowls. Jap. J. Zootech. Sci. 52, 10, 713.
• Hillel J., Korol A., Kirzner V., Freidlin P., Weigend S., Barre-Dirie A.. Groenen A. M. Crooijmans R. P. M. A. Tixier-Boichard M., Vignal A., Wimmers K., Burke T., Thomson P. A., Maki-Tanila A., Elo K., Zhivotovsky L.A., Feldman M.W., 1998. Biodiversity of chickens based on DNA pools: first results of the EC funded project AVIANDIV. Proc. 10th European Poultry Conf. Jerusalem. Israel 21-26 June1998. Israel Branch, 22-29.
• Inafuku K., Maeda Y., Okamoto S., Ardiningsasi S. M., Hashiguchi T., 1998. Polymorphism of egg white proteins in native chickens in Indonesia. Jap. Poult. Sci. 35, 5, 278-284.
• Moiseeva I. G., Voloknovich V.A., Tolokonnikova E.V., Altukhov Yu.P., 1984. Differentiation of chicken breeds for biochemical gene markers. Gienietika 4, 671-681.
• Nei M., 1978. The theory of genetic distance and evolution of human races. Jap. J. Hum. Genet. 23, 341-369.
• O’Brien S. J., 1991. Mammalian genome mapping: lessons and prospects. Current Opinions in Genetics and Development 1, 105-111.
• Pepin L., Nguyen T. C., 1994. Blood groups and protein polymorphisms in five goat breeds (Capra hircus). Anim. Genet. 25, 333-336.
• Plotsky Y., Kaiser M. G., Lamont S.J., 1995. Genetic characterization of highly inbred chicken lines by two DNA methods: DNA fingerprinting and polymerase chain reaction using arbitrary primers. Anim Genet. 26, 163-170.
• Weigend S., Romanov M. N., 2001. Current strategies for assessment and evaluation of genetic diversity in chicken resources. World`s Poult. Sci. J. 57, 3, 275-288.
• Wężyk S., Cywa-Benko K., Siwek M., Bednarczyk M., Calik J., 2000. Studies on genetic variability of conservation breeds of chickens [Badania nad genetycznym zróżnicowaniem zachowawczych ras kur]. Rocz. Nauk. Zootech. 27, 1, 115-127 [in Polish].
• Zhang X., Mc. Daniel G. R., Glambrone J. J., 1995. Random Amplified Polymorphic DNA comparison among broiler lines selected for incidence of tibial dyschondroplasia. Poult. Sci. 74, 1253-1258.