Easy PCR screening of Pichia pastoris transformants

• Autorzy: Arora D. , Chauhan A. , Khanna N.
• Języki publikacji: EN

Abstrakty

EN

A simple protocol for easy PCR screening of P. pastoris transformants is described. In short, the P. pastoris cells are lysed with very small amount of the enzyme Zymolase and the crude cell lysate is directly used in PCR. This protocol needs no tube transfer steps and also obliviates the requirement of freezing the samples at -80°C before PCR screening. Because of a single step screenig, both overall and actual hands on time are considerably reduced.

Słowa kluczowe

EN

zymolase; screening; enzyme; Pichia pastoris; lysate; polymerase chain reaction

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 1998
• Tom: 03
• Numer: 1
• Opis fizyczny: p.21-24,fig.

Twórcy

autor

Arora D.

• International Centre for Genetic Engineering and Biotechnology, P.O.Box 10504, Aruna Asaf Ali Marg, New Delhi - 110 067, India

autor

Chauhan A.

autor

Khanna N.

Bibliografia

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