Application of different diagnostic methods for the detection of SVDV infection in pigs

• Autorzy: Niedbalski W
• Języki publikacji: EN

Abstrakty

EN

The period of swine vesicular disease (SVD) virus persistence in clinical and tissue samples from experimentally infected pigs was investigated. A range of samples including epithelial tissue from vesicles, nasal swabs, blood, faeces and organs were collected to examine for the presence of infectious SVD particles, genomes and antigen. For this purpose, the conventional and PCR techniques were applied. The RT-nPCR assay appeared to be the most sensitive for the detection of SVDV in samples taken late in the course of infection. Only by nested PCR we could found the presence of vRNA in blood and nasal swabs as long as 4 and 48 d.p.i. , respectively. Using virus isolation and RT-nPCR it was possible to detect viral genome in faeces up to 70 d.p.i. By RT-nPCR, the vRNA could be detected in somatic muscles and tonsil till 25 and 48 d.p.i., respectively. The virus could not generally be found in other organs beyond 7 d.p.i. The described RT-nPCR procedure can be useful for the duration estimation of infection of pigs with SVDV.

Słowa kluczowe

EN

pig; Picornaviridae; virus; diagnostic method; swine vesicular disease; clinical sample; detection

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 1999
• Tom: 43
• Numer: 1
• Opis fizyczny: p.11-18,fig.

Twórcy

autor

Niedbalski W

• National Veterinary Research Institute, 98-220 Zdunska Wola, Poland

Bibliografia

• 1. Brocchi E., Berlinzani A., Gamba D., De Simone F.: J. virol. Methods 52, 155, 1995.
• 2. Burrows R., Mann J.A., Goodridge D.: J. Hyg., Camb. 72, 135, 1974.
• 3. Burrows R., Greig A., Goodridge D.: Res. vet. Sci. 15, 141, 1973.
• 4. Frey B., Suppmann B.: Biochemica 2, 34, 1995.
• 5. Hofner M.C., Carpenter W.C., Ferris N.P., Kitching R.P., Ariza Botero F.: J. virol. Methods 50. 11, 1994.
• 6. Hummeler K., Hamparian V.: Science 125, 547, 1957.
• 7. Kęsy A., Niedbalski W., Fitzner A., Paprocka G.: Medycyna wet. 53, 270, 1997.
• 8. Knowles N.J., Sellers R.F.: Swine vesicular disease. In: G.W. Beran (Ed), Handbook of Zoonoses. 2nd Edn., Section B: Viral. CRC Press, Boca Raton, pp. 437-444, 1994.
• 9. Knowles N.J.: www.iah.bbsrc.uk/virus/Picornaviridae/Enterovirus/svdvdist.htm
• 10. Lin F., Mackay D.K.J., Knowles N.J.: J. virol. Methods 65, 111, 1997.
• 11. Mann J.A., Burrows., Goodridge D.: Bull. Off. int. Epizoot. 83 , 117, 1975.
• 12. Mann J.A., Burrows R., Goodridge D.: XVI Confer. OIE Commission on FMD, Paris. 11-14 March 1975. Raport nr 505.
• 13. Nardell L., Lodetti G., Gualandi G., Goodrodge D., Brown F., Cartwright B.: Nature 219, 1275, 1968.
• 14. Niedbalski W., Kęsy A., Paprocka G., Fitzner A.: Bull. vet. Inst. Pulawy 39, 85, 1995.
• 15. Niedbalski W., Kęsy A., Paprocka G., Fitzner A.: Instrukcja badania laboratoryjnego w kierunku pryszczycy i choroby pęcherzykowej świń (SVD), Instrukcja nr 2/94 Min. Roln. Gosp. Żywn., 1994.
• 16. Niedbalski W., Kęsy A., Paprocka G., Fitzner A.: Medycyna wet. 54, 622, 1998.
• 17. Niedbalski W.: Bull. vet. Inst. Pulawy 42, 3, 1998.
• 18. OIE, Disease lnformation.: www.oie.int/Info/a_dsum.htm
• 19. Pussant C., Houdebine L.M. Biotechniques 8, 148, 1990.
• 20. Secchurn P., Knowles N.J., MCCauley J.W.: Virus Res. 16, 255, 1990.
• 21. Shieh Y-S.C., Wait D., Tai L., Sobsey M.D.: J. virol. Methods 54, 51, 1995.
• 22. Wiśniewski J.: Habil. dissertation, Veterinary Institute, Pulawy , 1978.
• 23. Zoll G.J., Melchers W.J.G., Kopecka H., Jambroes G., van der Poel H.J.A., Galama J.M.D.: J. clin. Microbiol. 30, 160, 1992