Wplyw permetryny i DDT na aktywnosc form molekularnych cytochromu P-4501A i 2B w watrobie szczura

• Autorzy: Kostka G. , Palut D. , Wiadrowska B.
• Języki publikacji: PL

Abstrakty

PL

Porównano wpływ pestycydów: DDT i permetryny na indukcję form molekularnych cytochromu Р-4501A i 2B. Stwierdzono, że permetrynę podobnie jak DDT; zaliczyć można do induktorów cytochromu P-4502B-typu fenobarbitalu.

EN

The effect of permethrin on relative liver weight (RLW) and the activity of hepatic monooxygenase system related to cytochrome P-4502B and 2A was studies. The effect of permethrin was compared with DDT used as phenobarbital-type of monooxygenase inducer (induces cyt. P-4502B). Male Wistar rats received permethrin and DDT for 4 days at 24 h intervals in daily oral doses of 1/10, 1/50 and 1/100 LD50. 3-methylocholantrene and phenobarbital which served as inducers of cytochrome P-4501A and 2B, respectively and were used as positive controls. The activities of cytochrome(s) P-450 were measured by 7-pentoxy- and 7-etoxyresofurin O-dealkylation by S-9 fraction of rat liver; these two compounds have been shown to be the substrates for reactions mediated by cytochrome P-4502B and 2A. Thus this biochemical procedure permits to determine whether tested compound belongs to one of two main types of inducers of the cytochrome P-450 monoxygenase system. Treatment of rats with both pesticides resulted in significant increase in RLW, to 30 and 15% of control, respectively. In animals treated with permethrin the metabolism of 7-pentoxyresofurin increased in a dose dependent manner. Phenobarbital and the highest dose of permethrin (620 mg/kg b.w. x day-1) induced similar (about 30-fold) increase in O-dealkylation of 7-pentoxyresofurin. DDT stimulated metabolism of 7-pentoxyresofurin to much higher degree as compared with phénobarbital. It should be noted that both pesticides induced only slight increase in O-dealkylation of 7-etoxyresofurin (cyt. P-4501A-mediated reaction). The present results indicate that permethrin as well as DDT shows the ability to induce the phenobarbital-type of cytochrome P-4502B.

Słowa kluczowe

PL

metabolizm zwierzat; szczury; cytochrom P-450; zwierzeta laboratoryjne; permetryna; DDT; watroba; indukcja; zwiazki chemiczne

EN

animal metabolism; rat; cytochrome P450; laboratory animal; permethrin; DDT; liver; induction; chemical compound

• Wydawca: -
• Czasopismo: Roczniki Państwowego Zakładu Higieny
• Rocznik: 1997
• Tom: 48
• Numer: 3
• Opis fizyczny: s.229-237,tab.,wykr.,bibliogr.

Twórcy

autor

Kostka G.

• Panstwowy Zaklad Higieny, 00-791 Warszawa, ul.Chocimska 24

autor

Palut D.

autor

Wiadrowska B.