Disruption of Trichoderma reesei gene encoding protein O-mannosyl-transferase I results in a decrease of the enzyme activity and alteration of cell wall composition

• Autorzy: Gorka-Niec W. , Pniewski M. , Kania A. , Perlinska-Lenart U. , Palamarczyk G. , Kruszewska J.S.
• Języki publikacji: EN

Abstrakty

EN

In fungi transfer of the first mannosyl residue to proteins during their O-glycosylation is catalyzed by protein O-mannosyltransferases encoded by pmt genes. Disruption of the pmt1gene in Trichodermacaused a significant decrease in the total activity of protein O-mannosyltransferases. Moreover, disruption of the pmt1gene also led to osmotic sensitivity of the strain, indicating an essential role of the PMTI protein activity for cell wall synthesis. At the same time, the strain was defective in septa formation, producing only half the number of septa per unit length of hypha compared with the wild type. Disruption of the pmt1gene decreased protein secretion but had no effect on glycosylation of secreted proteins, which suggests that PMTI protein O-mannosyltranferase does not take part in glycosylation of these proteins.

Słowa kluczowe

PL

Trichoderma reesei; disruption; gene encoding; pmt1 gene; protein glycosylation; cell wall composition; fungi; enzyme activity; alteration; mannosyl residue; protein; protein O-mannosyltransferase

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2008
• Tom: 55
• Numer: 2
• Opis fizyczny: p.251-259,fig.,ref.

Twórcy

autor

Gorka-Niec W.

• Polish Academy of Sciences, A.Pawinskiego 5a, 02-106 Warsaw, Poland

autor

Pniewski M.

autor

Kania A.

autor

Perlinska-Lenart U.

autor

Palamarczyk G.

autor

Kruszewska J.S.

Bibliografia

• Agaphonov MO, Sokolov S, Romanova NV, Sohn J, Kim SY, Kalebina TS, Choi ES, Ter-Avanesyan MD (2005) Mutation of the protein-O-mannosyltransferase enhances secretion of the human urokinase-type plasminogen activator in Hansenula polymorpha. Yeast 22:1037–1047.
• Carlos J, Cortes G, Konomi M, Martins IM, Munoz J, Moreno B, Osumi M, Duran A, Ribas JC (2007) The (1,3)β-d-glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation. Mol Microbiol 65: 201–217.
• da-Silva MM, Polizeli ML, Jorge JA, Terenzi HF (1994) Cell wall deficiency in “slime” strains of Neurospora crassa: osmotic inhibition of cell wall synthesis and beta-d-glucan synthase activity. Braz J Biol Res 27: 2843–2857.
• Dubois M, Gilles K, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugar and related substrates. Anal Biochem 28: 350–356.
• Ecker M, Mrsa V, Hagen I, Deutzmann R, Strahl S, Tanner W (2003) O-mannosylation precedes and potentially controls the N-glycosylation of a yeast cell wall glycoprotein. EMBO Rep 4: 628–632.
• Gentzsch M, Tanner W (1996) The PMTgene family: protein O-glycosylation in Saccharomyces cerevisiae is vital. EMBO J 15: 5752–5759.
• Gentzsch M, Tanner W (1997) Protein-O-glycosylation in yeast: protein-specific mannosyltransferases. Glycobiology 7:481–486.
• Górka-Nieć W, Bańkowska R, Palamarczyk G, Krotkiewski H, Kruszewska JS (2007) Protein glycosylation in pmt mutants of Saccharomyces cerevisiae. Influence of heterologously expressed cellobiohydrolase II of Trichoderma reesei and elevated levels of GDP-mannose and cisprenyltransferase activity. Biochim Biophys Acta 1770: 774–780.
• Gruber F, Visser J, Kubicek CP, de Graaff LH (1990) Cloning of the Trichoderma reesei pyrGgene and its use as a homologous marker for a high-frequency transformation system. Curr Genet 18: 447–451.
• Harman GE, Kubicek CP (1998) Enzymes, biocontrol and commercial applications. In Trichoderma and Glocladium. Harman EG, Kubicek CP, eds, vol. 2, pp 129–163. Taylor and Francis Ltd., London.
• Kruszewska J, Messner R, Kubicek CP, Palamarczyk G (1989) O-glycosylation of proteins by membrane fraction of Trichoderma reesei QM9414. J Gen Microbiol 135: 301–307.
• Kruszewska J, Butterweck AH, Kurzątkowski W, Migdalski A, Kubicek CP, Palamarczyk G (1999) Overexpression of the Saccharomyces cerevisiae mannosylphos phodolichol synthase — encoding gene in Trichoderma reesei results in an increased level of protein secretion and abnormal cell ultrastructure. Appl Environm Microbiol 65:2382–2387.
• Levin DE (2005) Cell wall integrity signaling in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 69: 262–291.
• Lommel M, Bognat M, Strahl S (2004) Aberrant processing of the WSC family and Mid2p cell surface sensors in cell death of Saccharomyces cerevisiae O-mannosylation mutants. Mol Cell Biol 24: 46–57.
• Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275.
• Nemcovic M, Farkas V (2001) Cell wall composition and polysaccharide synthase activity changes following photoinduction in Trichoderma viride. Acta Biol Hung 52: 281–288.
• Oka T, Hamaguchi T, Sameshima Y, Goto M, Furukawa K (2004) Molecular characterization of protein O-mannosylation and its involvement in cell-wall synthesis in Aspergillus nidulans. Microbiol 150: 1973–1982.
• Oka T, Sameshima Y, Koga T, Kim H, Goto M, Furukawa K (2005) Protein O-mannosyltransferase A of Aspergillus awamori is involved in O-mannosylation of glucoamylase I. Microbiol 151: 3657–3667.
• Palamarczyk G, Hemming FW (1975) The formation of mono-N-acetylhexosamine derivatives of dolichol phosphate by pig liver microsomal fractions. Biochem J 148: 243–251.
• Palamarczyk G, Maras M, Contreras R, Kruszewska J (1998) Protein secretion and glycosylation in Trichoderma. In Trichoderma and Glocladium.
• Kubicek CP, Harman GE, eds, vol 1, pp 121–138. Taylor and Francis Ltd, London.
• Perlińska-Lenart U, Kurzątkowski W, Janas P, Kopińska A, Palamarczyk G, Kruszewska JS (2005) Protein production and secretion in an Aspergillus nidulansmutant impaired in glycosylation. Acta Biochim Polon 52: 195–205.
• Perlińska-Lenart U, Bańkowska R, Palamarczyk G, Kruszewska JS (2006a) Overexpression of the Saccharomyces cerevisiae RER2gene in Trichoderma reeseiaffects dolichol dependent enzymes and protein glycosylation. Fungal Genet Biol 43: 422–429.
• Perlińska-Lenart U, Orłowski J, Laudy AE, Zdebska E, Palamarczyk G, Kruszewska JS (2006b) Glycoprotein hypersecretion alters the cell wall in Trichoderma reesei strains expressing the Saccharomyces cerevisiae dolichyl-phosphate mannose synthase gene. Appl Environ Micro-biol 72: 7778–7784.
• Pless DD, Palamarczyk G (1987) Comparison of polyprenol derivatives in yeast glycosyl transfer reactions. Biochim Biophys Acta 529:21–28.
• Reissig JL, Storminger JL, Leloir LF (1955) A modified colorimetric method for the estimation of N-acetylami-no sugars. J Biol Chem 217: 959–966.
• Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn, vol 1, pp 7.37–7.52. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
• Sharma CB, Babczinski P, Lehle L, Tanner W (1974) The role of dolicholmonophosphate in glycoprotein bio-synthesis in Saccharomyces cerevisiae. Eur J Biochem46: 35–41.
• Strahl-Bolsinger S, Gentzsch M, Tanner W (1999) Protein O-mannosylation. Biochim Biophys Acta1426: 297–307.
• Willer T, Brandl M, Sipiczki M, Strahl S (2005) Protein O-mannosylation is crucial for cell wall integrity, sep-tation and viability in fission yeast. Mol Microbiol57: 156–170.
• Yanish-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide se-quences of the M13mp18 and pUC 19 vectors. Gene 33: 103–119.
• Zakrzewska A, Migdalski A, Saloheimo M, Penttilä ME, Palamarczyk G, Kruszewska JS (2003a) cDNA encoding protein O-mannosyltransferase from the filamen-tous fungus Trichoderma reesei; functional equivalence to Saccharomyces cerevisiae PMT2. Curr Genet 43: 11–16.
• Zakrzewska A, Palamarczyk G, Krotkiewski H, Zdebska E, Saloheimo M, Penttilä M, Kruszewska JS (2003b) Over - expression of the gene encoding GTP-mannose-1–phos -phate guanyltransferase, mpg1, increases cellular GDP-mannose levels and protein mannosylation in Trichode - rma reesei. Appl Environ Microbiol 69 : 4383–4389.