Multiple extraction method for DNA, RNA and nuclear protein isolation from peripheral blood

• Autorzy: Ferry A. E. , Pace B.S.
• Języki publikacji: EN

Abstrakty

EN

Obtaining adequate samples for nucleic acid or protein analysis from a limited number of cells can be a difficult task. The steps for isolation of DNA, cytoplasmic RNA and nuclear proteins from mononuclear cells collected from a single peripheral blood sample are outlined below. A previously described technique for rapid isolation of nuclear proteins was modified to acquire RNA and DNA of sufficient quantity and quality to perform analyses at the molecular level without altering the quality of protein extracted. This approach is applicable for use with peripheral blood mononuclear cells, primary cultures and immortalized cell lines.

Słowa kluczowe

EN

peripheral blood; nuclear protein; protein extraction; cell; DNA isolation; protein analysis; nucleic acid; RNA isolation; extraction method

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 1998
• Tom: 03
• Numer: 4
• Opis fizyczny: p.375-378,fig.

Twórcy

autor

Ferry A. E.

• University of South Alabama, Mobile, AL 36688, USA

autor

Pace B.S.

Bibliografia

• 1. Andrews, N. C. and Faller, D. V. Nucleic Acids Res. 19 (1991) 2499.
• 2. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Smith, J. A., Seidman, J. G. and Struhl, K. in: Current Protocols in Molecular Biology, Greene Publishing Associates and Wiley-Interscience, Brooklyn, NY, 1987, 2.2.1-2.2.3.
• 3. Ferry, A. E., Baliga, S. B., Monteiro, C. and Pace, B. S. J. Biol. Chem. 272 (1997) 20030-20037.