Wlasciwosci adhezyjne i lekoopornosc szczepow Klebsiella izolowanych z przewodu pokarmowego dzieci hospitalizowanych we Wroclawiu i Opolu

• Autorzy: Maczynska B. , Smutnicka D. , Matusiewicz K. , Przondo-Mordarska A. , Burdynowski K.
• Języki publikacji: PL

Abstrakty

PL

Badano lekooporność, aktywność hemaglutynacyjną i adherencję do linii komórkowych szczepów Klebsiella izolowanych z przypadków biegunek od dzieci hospitalizowanych w oddziałach pediatrycznych we Wrocławiu i w Opolu. Stwierdzono występowanie szczepów wieloopornych, których odsetek był szczególnie wysoki w oddziale opolskim. Zdolności adhezyjne szczepów Klebsiella pochodzących z przewodu pokarmowego określono jako bardzo wysokie.

EN

Gram negative Klebsiella bacilli present many pathogenic properties, which determine their ability to survive and rapid spreading in hospital environment. There are many factors responsible for the pathogenicity of Klebsiella strains: capsule, fimbriae, nonfimbrial adhesins, lipopolysaccharide of the cell wall and extracellular secreted exotoxins. Klebsiella strains are etiological agents of different nosocomial infections but also colonized gastrointestinal and respiratory tracts. The aim of our work were adhesive properties and antibiotic resistance of Klebsiella strains isolated from stool of hospitalized children, according to source of potential nosocomial infections - 100 Klebsiella strains from Wroclaw and 76 strains from Opole, isolated in cases of diarrhea. The resistance of this strains to different group of antibiotics, the expression of ESBL enzymes, the activity in hemagglutination and their ability to adherence to different cell lines were tested. The highest resistance of all strains to aminopenicillins was observed. The production of ESBL was highest in strains from Opole (51% strains) then in Wroclaw (9%). In both hospital units, ESBL+ strains were resistant to aminoglicosides and Cotrimoxazol but sensitive to Ciprofloxacine. Using hemagglutination method the types of fimbriae were defined. Above 90 % investigated Klebsiella strains showed the presence of fimbriae (in Wroclaw more strains simultaneously expressed fimbriae type 1 and 3, in Opole mainly fimbriae type 3). Over 70 % strains demonstrated the high level of adherence to cell lines. Only several strains showed the low level or the lack of adhesion. These results suggested that among Klebsiella strains in gastrointestinal tract were presented multiresistant strains with high ability to adherence, which may be potential source of nosocomial infections.

Słowa kluczowe

PL

lekoopornosc; szpitale; przewod pokarmowy; chorobotworczosc; Wroclaw; Opole; choroby czlowieka; pacjenci; biegunki; dzieci; wlasciwosci adhezyjne; zakazenia szpitalne; Klebsiella

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2003
• Tom: 55
• Numer: 4
• Opis fizyczny: s.333-342,tab.,fot.,bibliogr.

Twórcy

autor

Maczynska B.

• Akademia Medyczna, Wroclaw

autor

Smutnicka D.

autor

Matusiewicz K.

autor

Przondo-Mordarska A.

autor

Burdynowski K.

Bibliografia

• 1. Alvarez D, Merino S, Thomas J, i inni. Capsular polysaccharide is a major complement resistance factor in lipopolysaccharide O side chain-deficient Klebsiella pneumoniae clinical isolates. Infect Immun 2000; 68: 953-5.
• 2. Baraniak A, Fiett J, Sulikowska A i inni. Countrywide spread of CTX-M-3 extended-spectrum ß-laktamase-producing microorganisms of the family Enterobacteriaceae in Poland. Antimicrob Agents Chemother 2002; 46: 151-9.
• 3. Darfeuille-Michaud A, Jallat C, Aubel D i inni. R-plasmid-encoded adhesive factor in Klebsiella pneumoniae strains responsible for human nosocomial infections. Infect Immun 1992; 60: 44-55.
• 4. Di Martino P, Livrelli V, Sirot D i inni. New fimbrial antygen harbored by CAZ-5/SHV-4-producing Klebsiella pneumoniae strains involved in nosocomial infections. Infect Immun 1996; 64: 2266-73.
• 5. Di Martino P, Sirot D, Joly В і inni. Relationship between adhesion to Intestinal Caco-2 cells and multidrug resistance in Klebsiella pneumoniae clinical isolates. J Clin Microbiol 1997; 35: 1499-503.
• 6. Di Martino P, Bertin Y, Girardeau JP i inni. Molecular Characterization and Adhesive Properties of CF29K, an Adhesin of Klebsiella pneumoniae Strains Involved in Nosocomial Infections. Infect Immun 1995; 63: 4336-44.
• 7. Duguid JP, Old CD. Adhesive properties of Enterobacterieceae. Bacterial Adherence. E. Bachey, Chapman and Hall. London 1980; 187-215.
• 8. Favre-Bonte S, Darfeuille-Michaud A, Forestier C. Aggregative adherence of Klebsiella pneumoniae to human Intestine-407 cells. Infect Immun 1995; 63: 1318-28.
• 9. Fiett J, Palucha A, Mączyńska В i inni. A Novel Complex Mutant beta-Lactamase, Tem-68, Identified in a Klebsiella pneumoniae Isolate from an Outbreak of extended-Spectrum ß-Lactamase-Producing Klebsiellae. Antimicrob Agents Chemother 2000; 44: 1499-505.
• 10. Hansen DS, Mestre F, Alberti S, i inni. Klebsiella pneumoniae Lipopolysaccharide O-Typing Revision of Prototype Strains and O-Group Distribution among Clinical Isolates from Different Sources and Countries. J Clin Microbiol 1999; 37: 56-62.
• 11. Jones CH, Pinkner J S, Roth R i inni. FimH adhesin of type 1 pili is assembled into a fibrillar tip structure in the Enterobacteriaceae. Proc Natl Acad Sci USA. 1995; 92: 2081-5.
• 12. Klemm P, Shembri MA. Bacterial adhesins: function and structure. Int J Med Microbiol 2000; 290: 27-35.
• 13. Livermore DM, Yuan M. Antibiotic resistance and production of extended-spectrum beta-lactamases amongst Klebsiella spp. From intensive care units in Europe. J Antimicrob Chemother 1996; 38: 409-24.
• 14. Livrelli V, De Champs C, Di Martino A i inni. Adhesive properties and antibiotic resistance of Klebsiella, Enterobacter, and Serratia clinical isolates involved in nosocomial infections. J. Clin. Microbiol., 1996, 34(8); 1963-9.
• 15. Minami J, Saito S, Yoshida T i inni. Biological activities and chemical composition of a cytotoxin of Klebsiella oxytoca. J Gen Microbiol 1992; 138: 1921-7.
• 16. Podschun R, Sahly H. Hemagglutinins of Klebsiella pneumoniae and oxytoca isolated from different sources. Zbl Hyg Umweltmed 1991; 191: 46-52
• 17. Podschun R, Ullman U. Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin Microbiol Rev 1998; 11: 589-603.
• 18. Przondo-Mordarska A, Smutnicka D, Ko HL i inni. Adhesive Properties of P-like Fimbriae in Klebsiella-Species. Zbl Bakt 1996; 284: 372-7.
• 19. Przondo-Mordarska A, Gamian A, Ko HL i inni. Importance of Klebsiella fimbriae in adhesion. Zbl Bakt 1993; 25: 59-68.
• 20. Scaletsky ICA, Silva MLM, Trabulsi LR. Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells. Infect Immun 1984; 45: 534-6.
• 21. Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975; 98: 503
• 22. Staniszewska M, Witkowska D, Gamian A. Fimbrie jako czynnik patogenności bakterii i nośnik w szczepionkach koniugatowych. Post Hig Med Dośw 2000; 54: 727-47.
• 23. Tarkkanen A, Virkola R, Clegg S i inni. Binding of the type 3 fimbriae of Klebsiella pneumoniae to human endothelial and urinary bladder cells. Infect Immun 1997; 65: 1546-9.