Metody immortalizacji komorek i ich zastosowanie w badaniach nad rozrodem

• Autorzy: Bodek G , Blitek A. , Kowalczyk A.E. , Kiewisz J. , Ziecik A.J.

Warianty tytułu

EN

Methods of cell immortalization and their application in reproductive studies

• Języki publikacji: PL

Abstrakty

EN

After a few cell divisions primary cell cultures enter into a terminally nondividing state. Replicative senescence of cells involves a wide range of cellular events including changes in gene expression. Using immortalization methods it is possible to immortalize many of the primary cell cultures. The major advantage is that the immortalized cells represent the tissue of origin. It is possible to distinguish spontaneous and induced immortalization. The spontaneous immortalization of cultured cells is quite a rare event. Carcinogenic agents, radiation and viral oncogens can induce immortalization of cells. Transfection of cells with oncogens and telomerase activation generates an extended lifespan of cells. This review discusses various approaches to cell immortalization and the application of such cell lines for studies on animal reproduction.

Słowa kluczowe

PL

immortalizacja indukowana; hodowla komorek; immortalizacja spontaniczna; immortalizacja; rozrod zwierzat; transfekcja; metody; linie komorkowe; badania in vitro

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 04B
• Opis fizyczny: s.537-540,rys.,bibliogr.

Twórcy

autor

Bodek G

• Instytut Rozrodu Zwierzat i Badan Zywnosci PAN, ul.Tuwima 10, 10-747 Olsztyn

autor

Blitek A.

autor

Kowalczyk A.E.

autor

Kiewisz J.

autor

Ziecik A.J.

Bibliografia

• 1. Band V.: In vitro models of early neoplastic transformation of human mammary epithelial cells. Methods Mol. Biol. 2003, 223, 237-248.
• 2. Band V.: Preneoplastic transformation of human mammary epithelial cells. Semin. Cancer Biol. 1995, 6, 185-192.
• 3. Beausejour C. M., Krtolica A., Galimi F., Narita M., Lowe S. W., Yaswen P.: Reversal of human cellular senescence: roles of the p53 and p16 pathways. EMBO J. 2003, 22, 4212-4222.
• 4. Ben-Porath I., Weinberg R. A.: When cells get stressed: an integrative view of cellular senescence. Review. J. Clin. Invest. 2004, 113, 8-13.
• 5. Blitek A., Waclawik A., Kaczmarek M. M., Stadejek T., Pejsak Z., Ziecik A. J.: Expression of cyclooxygenase-1 and -2 in the porcine endometrium during the oestrous cycle and early pregnancy. Reprod. Domest. Anim. 2006, 41, 251-257.
• 6. Blitek A., Ziecik A. J.: Effect of LH on prostaglandin F2alpha and prostaglandin E2 secretion by cultured porcine endometrial cells. Reproduction 2005, 130, 105-112.
• 7. Bodnar A. G., Ouellette M., Frolkis M., Holt S. E., Chiu C. P., Morin G. B.: Extension of life-span by introduction of telomerase into normal human cells. Science 1998, 279, 349-352.
• 8. Bowen J. A., Newton G. R., Weise D. W., Bazer F. W., Burghardt R. C.: Characterization of a polarized porcine uterine epithelial model system. Biol. Reprod. 1996, 55, 613-619.
• 9. Campisi J.: The biology of replicative senescence. Eur. J. Cancer 1997, 33, 703-709.
• 10. Cussenot O., Teillac P., Le Duc A., Ketels F., Calvo F.: Culturing of normal and tumor cells of the human prostate. Ann. Urol. (Paris) 1989, 23, 533-537.
• 11. Forsythe N. R., Evans A. P., Shay J. W., Wright W. E.: Developmental differences in the immortalization of lung fibroblasts by telomerase. Aging Cell 2003, 2, 235-243.
• 12. Glasser S. R., Bazer F. W., Wang G.: ICI 182 870 produces mixed antiestrogeneffects on polarized pregnant pig uterine luminal epithelialcells. Biol. Reprod. 1998, 58, 173.
• 13. Goyal J., Smith K. M., Cowan J. M., Wazer D. E., Lee S. W., Band V.: The role for NES1 serine protease as a novel tumor suppressor. Cancer Res. 1998, 58, 4782-4786.
• 14. Gudjonsson T., Villadsen R., Nielsen H. L., Ronnov-Jessen L., Bissell M. J., Petersen O. W.: Isolation, immortalization and characterization of a human breast epithelial cell line with stem cell properties. Genes Dev. 2002, 16, 693-706.
• 15. Halliwell B.: Oxidative stress in cell culture: an underappreciated problem? FEBS Lett. 2003, 540, 3-6.
• 16. Hausen H.: Molecular pathogenesis of cancer of the cervix and its causation by specific human papillomavirus types. Curr. Top. Microbiol. Immunol. 1994, 186, 131-156.
• 17. Johnson G. A., Burghardt R. C., Newton G. R., Bazer F. W., Spencer T. E.: Development and characterization of immortal ovine endometrial cell lines. Biol. Reprod. 1999, 61, 324-1330.
• 18. Klingelhutz A. J.: The roles of telomeres and telomerase in cellular immortalization and the development of cancer. Anticancer Res. 1999, 19, 4823-4830.
• 19. Kyo S., Nakamura M., Kiyono T., Maida Y., Kanaya T., Tanaka M., Yatabe N., Inoue M.: Successful immortalization of endometrial glandular cells with normal structural and functional characteristics. Am. J. Pathol. 2003, 163, 2259-2269.
• 20. Liu X. L., Wazer D. E., Watanabe K., Band V.: Identification of a novel serine protease-like gene, the expression of which is down-regulated during breast cancer progression. Cancer Res. 1996, 56, 3371-3379.
• 21. McGrath C. M., Soule H. D.: Calcium regulation of normal human mammary epithelial cell growth in culture. In Vitro 1984, 20, 652-662.
• 22. Miller F. R., Soule H. D., Tait L., Pauley R. J., Wolman S. R., Dawson P. J.: Xenograft model of progressive human proliferative breast disease. J. Natl. Cancer Inst. 1993, 85, 1725-1732.
• 23. Park C. C., Henshall-Powell R. L., Erickson A. C., Talhouk R., Parvin B., Bissell M. J.: Ionizing radiation induces heritable disruption of epithelial cell interactions. Proc. Natl. Acad. Sci. USA 2003, 100, 10728-10733.
• 24. Santner S. J., Dawson P. J., Tait L., Soule H. D., Eliason J., Mohamed A. N.: Malignant MCF10CA1 cell lines derived from premalignant human breast epithelial MCF10AT cells. Breast Cancer Res. Treat. 2001, 65, 101-110.
• 25. Shay J. W., Tomlinson G., Piatyszek M. A., Gollahon L. S.: Spontaneous in vitro immortalization of breast epithelial cells from a patient with Li-Fraumeni syndrome. Mol. Cell. Biol. 1995, 15, 425-432.
• 26. Shay J. W., Van Der Haegen B. A., Ying Y., Wright W. E.: The frequency of immortalization of human fibroblasts and mammary epithelial cells transfected with SV40 large T-antigen. Exp. Cell Res. 1993, 209, 45-52.
• 27. Soule H. D., Maloney T. M., Wolman S. R., Peterson W. D. Jr, Brenz R., McGrath C. M.: Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 1990, 50, 6075-6086.
• 28. Wang G., Johnson G. A., Spencer T. E., Bazer F. W.: Isolation, immortalization, and initial characterization of uterine cell lines: an in vitro model system for the porcine uterus. In Vitro Cell Dev Biol Anim. 2000, 36, 650-656.
• 29. Weaver V. M., Petersen O. W., Wang F., Larabell C. A., Briand P., Damsky C.: Reversion of the malignant phenotype of human breast cells in three-dimensional culture and in vivo by integrin blocking antibodies. J. Cell Biol. 1997, 137, 231-245.
• 30. Yaswen P., Stampfer M. R.: Molecular changes accompanying senescence and immortalization of cultured human mammary epithelial cells. Int. J. Biochem. Cell Biol. 2002, 34, 1382-1394.