Application of the ELISA for determination of anti-Pasteurella multocida IgG in the sera of rabbits veccinated against pasteurellosis under field conditions

• Autorzy: Borkowska-Opacka B , Kedrak A. , Truszczynski M.
• Języki publikacji: EN

Abstrakty

EN

The ELISA was used to measure the level of specific lgG to P. multocida in the sera of rabbits vaccinated against pasteurellosis on a farm at Ch. The vaccines comprised the antigens of serotypes 3 and 12 with two adjuvants: emulsigen or aluminium hydroxide gel. Eight-week-old rabbits were injected subcutaneously with a 1-ml dose. Animals were observed for about 2 months until slaughter. A statistically significant increase in IgG level was found in rabbits challenged one month after immunisation in comparison to non-vaccinated animals. The increase was maintained without any marked alterations for 2 months. Results indicate that the ELISA may be used to evaluate the vaccine effectiveness instead of experimental injections of animals.

Słowa kluczowe

EN

prevention; vaccination; field condition; serum; controlling; ELISA test; Pasteurella multocida; pasteurellosis; animal disease; rabbit

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 1997
• Tom: 41
• Numer: 1
• Opis fizyczny: p.17-24,fig.

Twórcy

autor

Borkowska-Opacka B

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kedrak A.

autor

Truszczynski M.

Bibliografia

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