Renal tissue NO and intrarenal haemodynamics during experimental variations of NO content in anaesthetised rats

• Autorzy: Grzelec-Mojzesowicz M. , Sadowski J.
• Języki publikacji: EN

Abstrakty

EN

Direct renal nitric oxide (NO) measurements were infrequent and no simultaneous measurements of renal cortical and medullary NO and local perfusion were reported. Large-surface NO electrodes were placed in renal cortex and medulla of anaesthetised rats; simultaneously, renal blood flow (RBF, index of cortical perfusion) and medullary laser-Doppler flux (MBF) were determined. NO synthase inhibitors: nonselective (L-NAME) or selective for neuronal NOS (nNOS) (S-methyl-thiocitrulline, SMTC), and NO donor (SNAP), were used to manipulate tissue NO. Baseline tissue NO was significantly higher in medulla (703±49 nM) than in cortex (231±17 nM). Minimal cortical and medullary NO current measured after maximal L-NAME dose (2.4 mg kg-1 i.v.) was taken as tissue NO zero level. This dose decreased RBF and MBF significantly (-43%). SMTC, 1.2 mg kg-1 h-1 i.v., significantly decreased tissue NO by 105±32 nM in cortex and 546±64 nM in medulla, RBF and MBF decreased 30% and 20%, respectively. Renal artery infusion of SNAP, 0.24 mg kg-1 min-1 significantly increased tissue NO by 139±18 nM in cortex and 948±110 nM in medulla. Since inhibition of nNOS decreased medullary NO by 80% and MBF by 20% only, this isoform has probably minor role in the maintenance of medullary perfusion.

Słowa kluczowe

EN

nitric oxide synthase; nitric oxide; hemodynamics; renal medulla; renal blood flow; renal cortex; renal tissue; intrarenal circulation

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2007
• Tom: 58
• Numer: 1
• Opis fizyczny: p.149-163,fig.,ref.

Twórcy

autor

Grzelec-Mojzesowicz M.

• Polish Academy of Sciences, Pawinskiego 5, 02-160 Warsaw, Poland

autor

Sadowski J.