Role of rpoS in the regulation of glyoxalase III in Escherichia coli

• Autorzy: Benov L. , Sequeira F. , Beema A.F.
• Języki publikacji: EN

Abstrakty

EN

Methylglyoxal is an endogenous electrophile produced in Escherichia coli by the en­zyme methylglyoxal synthase to limit the accumulation of phosphorylated sugars. In enteric bacteria methylglyoxal is detoxified by the glutathione-dependent glyoxalase I/II system, by glyoxalase III, and by aldehyde reductase and alcohol dehydrogenase. Here we demonstrate that glyoxalase III is a stationary-phase enzyme. Its activity reached a maximum at the entry into the stationary phase and remained high for at least 20 h. An rpoS- mutant displayed normal glyoxalase I and II activities but was unable to induce glyoxalase III in stationary phase. It thus appears that glyoxalase III is regulated by rpoS and might be important for survival of non-growing E. coli cultures.

Słowa kluczowe

EN

stationary phase; methylglyoxal; regulation; Escherichia coli; glyoxalase

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2004
• Tom: 51
• Numer: 3
• Opis fizyczny: p.857-860,fig.,ref.

Twórcy

autor

Benov L.

• Kuwait University, P.O. Box 24923 Safat, 13110 Kuwait, Kuwait

autor

Sequeira F.

autor

Beema A.F.

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