RT-PCR in diagnosis of BVDV infection

• Autorzy: Polak M P , Zmudzinski J.F.
• Języki publikacji: EN

Abstrakty

EN

RT-PCR was evaluated as a diagnostic tool for BVDY detection. First, five sets of primers were checked for their diagnostic ability to amplify the number of field isolates. Only primers within the 5'UTR region amplified all field isolates as well as all reference laboratory strains. Next, sensitivity of the test was evaluated. This was equal to 1 TCID₅₀ of the virus. lt was concluded that RT-PCR can be used as an alternative diagnostic test for inconclusive results of virus isolation in cell culture especially for the noncytopathic strains. However, high cost of equipment and reagents for the test precludes it as a routine diagnostic method except for research applications.

Słowa kluczowe

EN

infection; pathogen; cattle; diagnosis; polymerase chain reaction; diarrhea; bovine viral diarrhea virus

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 1999
• Tom: 43
• Numer: 2
• Opis fizyczny: p.113-118,fig.

Twórcy

autor

Polak M P

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Zmudzinski J.F.

Bibliografia

• 1. Alansari H., Brock K.V., Potgieter L.N.D.: J. vet. Diagn. lnvest. 5, 148, 1993.
• 2. Baker J.C.: Vet. Clin. North Am. 11, 425, 1995.
• 3. Bolin S.R., Mattews P.J., Ridpath J.F.: J. vet. diagn. lnvest. 3, 199, 1991.
• 4. Boye M., Kamstrup S., Dalsgaard K.: Vet. Microbiol. 29, 1, 1991.
• 5. Brock K.V., Potgieter L.N.: Vet. Microbiol. 24, 297, 1990.
• 6. Canal C.W., Hotzel I., de Almeida L.L., Roehe P.M., Masuda A.: Vet. Microbiol. 48, 373, 1996.
• 7. Chomczynski P., Sacchi N.: Anal. Biochem. 162, 156, 1987.
• 8. Da Silva N., Zardoya R., Santurde G., Solana A., Castro J.M.: J. virol. Meth. 55, 209, 1995.
• 9. Dubovi E.J.: Comp. lmmunol. Microbiol. lnfect. Dis. 15, 155, 1992.
• 10. Francki R., Fauquet C., Knudson D., Brown F.: Arch. Virol. (suppl. 2). Springer, New York, 1991, p.450.
• 11. Hamel A.L., Wasylyshen M.D., Nayar G.P.S: J. clin. Microbiol. 33, 287, 1995.
• 12. Laamanen U.I., Neuvonen E.P., Ylivuhkola E.M., Veijalainen P.M.L.: Res. vet. Sci. 63, 199, 1997.
• 13. Liu S.T., Li S.N., Wang D.C., Chang S.F., Chiang S.C., Ho W.C., Chang Y.S., Lai S.S.: J. virol. Meth. 35, 227, 1991.
• 14. Lopez O.J., Osorio F.A., Donis R.O.: J. clin. Microbiol. 29, 578, 1991.
• 15. Moussa A.: Proc. 3rd Congress Europ. Soc. vet. Virol. Edited by Schwyzer M. et al., 260, 1995.
• 16. Potgieter L.N.D., Brock K.V.: J. vet. diagn. Invest. 1, 29, 1989.
• 17. Reed L.J., Muench H.A.: Am. J. Hyg. 27, 493, 1938.
• 18. Ridpath J.F., Bolin S.R.: Mol. Cell. Probes 5, 291, 1991.
• 19. Ridpath J.F., Bolin S.R., Dubovi E.J.: Virology 205, 66, 1994.
• 20. Ridpath J.F., Bolin S.R., Katz J.: J. clin. Microbiol. 31, 986, 1993.
• 21. Sandvik T.: Vet. Microbiol. 64, 123, 1999.
• 22. Schroeder B.A., Balassu-Chan T.C.: Arch. Virol. 111, 239, 1990.
• 23. Vilcek S., Herring A.J., Herring J.A., Nettleton P.F., Lowings J.P., Paton D.J.: Arch. Virol. 136, 309, 1994.