Incorporation of stilbazolium merocyanines into human leukocytes measured by flow cytometry

• Autorzy: Wiktorowicz K. , Niedbalska M. , Planner A. , Frackowiak D.
• Języki publikacji: EN

Abstrakty

EN

Human peripheral blood leukocytes were incubated with thirteen various mero­cyanines of the stilbazolium betaine type and the fluorescence intensities of the cells were measured by flow cytometry. The fluorescence intensity of lymphocytes, mono­cytes and granulocytes depended on the time and temperature of incubation with the dyes. An increase in the incubation temperature enhanced the fluorescence intensity whereas washing of the cells after incubation had little influence on the observed emission. This points to incorporation of the dye molecules into the cell membrane. From the measured fluorescence intensities corrected for relative fluorescence yields, the relative efficiencies of incorporation into the cells of the various merocyanines tested were evaluated. The efficiency was dependent on the type of the cells and the length and side groups of the merocyanine molecules studied.

Słowa kluczowe

EN

tumour cell; fluorescence; flow cytometry; toxic effect; in vitro; leucocyte; human leucocyte; merocyanine

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 3
• Opis fizyczny: p.333-338,fig.

Twórcy

autor

Wiktorowicz K.

• K.Marcinkowski University School of Medical Sciences, Winogrady 144, 61-626 Poznan, Poland

autor

Niedbalska M.

autor

Planner A.

autor

Frackowiak D.

Bibliografia

• 1. Gruda, I., Page, M., Bolduc, F., Laliberte, S. & Noel, C. (1987) Merocyanines: potential new anticancer agents. Anticancer Res. 7,1125-1128.
• 2. Laliberte, S., Gruda, I., Page, ML, Granier, F., Pepin, A. & Noel, C. (1990) The effect of mole- cular structure in a series of merocyanines on the in vitro cytotoxicity for adenocarcinoma cells. Anticancer Res. 10, 939-942.
• 3. Niedbalska, M. & Gruda, I. (1992) Incorporation of stilbazolium merocyanines into biological membranes. Curr. Topics Biophys. 16,1-7.
• 4. Frąckowiak, D., Wiktorowicz, K., Cofta, J., Niedbalska, M. & Latosińska, M. (1995) Incorporation of stilbazolium merocyanines into resting and stimulated mononuclear leukocytes. Acta Biochim. Polon. 42, 61-68.
• 5. Gruda, I., Laliberte, S., Niedbalska, M. & Frąckowiak, D. (1987) Polarized spectra of series of stilbazolium merocyanines in poly­vinyl alcohol films.}. Lumin. 39,1-10.
• 6. Gruda, I. & Bolduc, F. (1984) Spectral properties and basicity of stilbazolium betaines containing bulky substituents on quinoid ring./. Org. Chem. 49,3300-3305.
• 7. Boyum, A. (1976) Isolation of lymphocytes, gra­nulocytes and macrophages. Scand. J. Immunol. 5,9-15.
• 8. Carter, N.P. & Meyer, E.W. (1990) Introduction to the principles of flow cytometry; in Flow cyto­metry. A practical approach (Ormerod, M.G., ed.) pp. 1-28, Oxford University Press, Oxford-New York-Tokyo.