Antagonism of higher fungi to Heterobasidion annosum (Fr.) Bref. in laboratory conditions

• Autorzy: Napierala-Filipiak A. , Werner A.

Warianty tytułu

PL

Badania nad antagonizmem pomiędzy grzybami mikoryzowymi a grzybem Heterobasidion annosum (Fr.) Bref. w warunkach in vitro

• Języki publikacji: EN

Abstrakty

EN

Interactions between 42 higher fungi and six strains of Heterobasidion annosum (Fr.) Bref., representing P and S intersterility groups were studied in vitro. Part of the higher fungi was represented by strains of ectomycorrhizal fungi indigenous to old disease centres caused by H. annosum in pine stands. Variation in antagonism to the pathogen was observed both within and between the species. The antagonism was dependent on growth rates of fungi in control. Fungi growing faster displayed a greater ability to arrest the development of the pathogen mycelia. The same tendency was observed in the strains of H. annosum, which generally grew faster and their growth was restricted less than that of higher fungi. Two strains of an ectedomycorrhizal fungus Mrg X, accompanied by a helper bacteria reduced mycelial extension of H. annosum by 72% and 76%. Among ectomycorrhizal fungi, the most effective antagonists were strains of Xerocomus subtomentosus, Amanita muscaria, A. citrina and Laccaria laccata. Three various types of interaction between the studied fungi were observed. Inhibition zone between interacting mycelia appeared often, however the fungi displayed this antagonistic property not to all strains of the pathogen. Although mycelia of H. annosum invaded colonies of the other fungi frequently, in none of the treatments growth of the higher fungus over the opposing colony of the pathogen was observed.

PL

W warunkach in vitro przebadano wpływ czterdziestu dwóch szczepów grzybów wyższych naogra niczanie wzrostu sześciu szczepów Heterobasidion annosum należących do dwóch intersterylnych grup P i S. Badania prowadzono według metody opisanej przez Marxa (1969). Grzyby hodowano w szalkach Petriego, na pożywce Pp (Pachlewski 1983). Po dwóch tygodniach mierzono powierzchnię kolonii obu grzybów (w cm2), strefę inhibicji (w mm) oraz opisano sytuację zaistniałą na szalce. Badane grzyby wyższe wykazywały znaczne zróżnicowanie stopnia ograniczania wzrostu grzyba H. annosum. Najlepszymi antagonistami w stosunku do sześciu badanych szczepów patogena okazały się szczepy grzyba ektendomikoryzowego oznaczonego symbolem Mrg X (18–1z i 19–1z), których grzybnie wykazywały obecność bakterii, szczep grzybaektomikoryzowego z gatunku Amanita muscaria (16–3), szczep grzybaniemikoryzowego Hygrophoropsis aurantiaca (55–1) oraz grzyba Phallus impudicus (26–1) nie tworzącego z sosną symbiozy. Obok zmienności międzygatunkowej stwierdzono dużą zmienność wewnątrzgatunkową. Różnice pomiędzy szczepami z gatunków: A. muscaria, Suillus luteus, Paxillus involutus czy H. aurantiaca były niekiedy bardziej znaczące niż pomiędzy gatunkami. Badane grzyby mikoryzowe cechowały się stosunkowo wolnym wzrostem na pożywce, natomiast większość badanych szczepów H. annosum wzrastała szybko. Stwierdzono zależność pomiędzy szybkością wzrostu a stopniem negatywnego oddziaływanianawzrost grzybów w bikulturach. Szczepy grzybów wyższych charakteryzujące się szybszym wzrostem przejawiały większą zdolność hamowania wzrostu szczepów grzyba H. annosum i jednocześnie wykazywały większą tolerancję na obecność patogena.

Słowa kluczowe

EN

Heterobasidion annosum; antagonism; mycorrhizal fungi; higher fungi; fungi; laboratory condition

• Wydawca: -
• Czasopismo: Dendrobiology
• Rocznik: 2000
• Tom: 45
• Opis fizyczny: p.65-81,fig.,ref.

Twórcy

autor

Napierala-Filipiak A.

• Polish Academy of Sciences, Parkowa 5, 62-035 Kornik, Poland

autor

Werner A.

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