Intranuclear trafficking of plasmid DNA is mediated by nuclear polymeric proteins lamins and actin

• Autorzy: Ondrej V. , Lukasova E. , Krejci J. , Kozubek S.
• Języki publikacji: EN

Abstrakty

EN

Functions of nuclear polymeric proteins such as lamin A/C and actin in transport of plasmid DNA were studied. The results show that the lamina plays an important role in plasmid DNA’s entry into the cell nucleus from the cytoplasm. Selective disruption of lamin A/C led to a halt in plasmid DNA transport through the nuclear envelope. Inside the nucleus, plasmid DNA was frequently localized at sites with impaired genome integrity, such as DNA double-strand breaks (DSBs), occurring spontaneously or induced by ionizing radiation. Polymeric actin obviously participates in nuclear transport of plasmid DNA, since inhibition of actin polymerization by latrunculin B disturbed plasmid transport inside the cell nucleus. In addition, precluding of actin polymerization inhibited plasmid co-localization with newly induced DSBs. These findings indicate the crucial role of polymeric actin in intranuclear plasmid transport

Słowa kluczowe

PL

Plasmid DNA; DNA double-strand break; nuclear actin; lamina; protein; actin; cytoplasm; cell nucleus; ionizing radiation

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2008
• Tom: 55
• Numer: 2
• Opis fizyczny: p.307-315,fig.,ref.

Twórcy

autor

Ondrej V.

• Palacky University, Slechtitelu 11, 783 71 Olomouc, Czech Republic

autor

Lukasova E.

autor

Krejci J.

autor

Kozubek S.

Bibliografia

• Bettinger BT, Gilbert DM, Amber DC (2004) Actin up in the nucleus. Nat Rev Mol Cell Biol 5: 410-415.
• Chubb JR, Boyle S, Perry P, Bickmore WA (2002) Chromatin motion is constrained by association with nuclear compartments in human cells. Curr Biol 12: 439-445.
• Clubb BH, Locke M (1998) Peripheral nuclear matrix actin forms perinuclear shells. J Cell Biochem 70:240-251.
• Dean DA (1997) Import of plasmid DNA into the nucleus is sequence specific. Exp Cell Res 230:293-302.
• Dowty ME, Williams P, Zhang G, Hagstrom JE, Wolff JA (1995) Plasmid DNA entry into postmitotic nuclei of primary rat myotubes. Proc Natl Acad Sci USA 92:4572-4576.
• Foster ER, Downs JA (2005) Histone H2A phosphorylation in DNA double-strand break repair. FEBS J 272: 3231-3240.
• Hawryluk-Gara LA, Shibuya EK, Wozniak RW (2005) Vertebrate Nup53 interacts with the nuclear lamina and is required for the assembly of a Nup93-containing complex. Mol Biol Cell 16:2382-2394.
• Hofmann W, Reichart B, Ewald A, Miller E, Schmidt I, Stauber RH, Lottspeich F, Jockusch BM, Scheer U, Gauner J, Dabauvalle MC (2001) Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs. An unexpected role for actin. J Cell Biol 152: 895-910.
• Hofmann W, Stojiljkovic L, Fuchsova B, Vargas GM, Mavrommatis E, Philimonenko V, Kysela K, Goodrich JA, Lessard JL, Hope TJ, Hozak P, de Lanerolle P (2004) Actin is part of pre-initiation complexes and is necessary for transcription by RNA polymerase II. Nat Cell Biol 6:1094-1101.
• Holaska JM, Kowalski AK, Wilson KL (2004) Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. PLoS Biol 2:e231.
• Kiseleva E, Drummond SP, Goldberg MW, Rutheford SA, Allen TD, Wilson KL (2004) Actin- and protein-4.1-containing filaments link nuclear pore complexes to subnuclear organelles in Xenopusoocyte nuclei. J Cell Sci 117:2481-2490.
• Kozubek M, Kozubek S, Lukasova E, Bartova E, Skalnikova M, Matula P, Matula P, Jirsova P, Cafourkova A, Koutna I (2001) Combined confocal and wide-field high-resolution cytometry of fluorescence in situ hybridization-stained cells. Cytometry 45: 1-12.
• Libotte T, Zaim H, Abraham S, Padmakumar VC, Schneider M, Lu W, Munck M, Hutchinson Ch, Wehnert M, Fahrenkrog B, Sauder U, Aebi U, Noegel AA, Karakesisoglou I (2005) Lamin A/C-dependent localization of nesprin-2, a giant scaffolder at the nuclear envelope. Mol Biol Cell 16:3411-3424.
• Lukacs GL, Haggie P, Seksek O, Lecherdeur D, Freedman N, Verkman AS (2000) Size-dependent DNA mobility in cytoplasm and nucleus. J Biol Chem 275: 1625-1629.
• Mattaj IW (2004) Sorting out the nuclear envelope from the endoplasmic reticulum. Nat Rev Mol Cell Biol 5:65-69.
• McDonald D, Carrero G, Andrin Ch, de Vries G, Hendzel MJ (2006) Nucleoplasmic β-actin exists in a dynamic equilibrium between low-mobility polymeric species and rapidly diffusing populations. J Cell Biol 172: 541-552.
• Mearini G, Nielsen PE, Fackelmayer FO (2004) Localization and dynamics of small circular DNA in live mammalian nuclei. Nucleic Acids Res 32: 2642-2651.
• Miller DG, Petek LM, Russell DW (2003) Human gene targeting by adeno-associated virus vectors is enhanced by DNA double-strand breaks. Mol Cell Biol 23: 3550-3557.
• Molas M, Gomez-Valadez AG, Vidal-Alabro A, Miguel-Turu M, Bermudez J, Bartrons R, Perales JC (2003) Receptor-mediated gene transfer vectors: Progress towards genetic pharmaceuticals. Curr Gene Ther 3: 468-485.
• Munkonge FM, Dean DA, Hillery E, Griesenbach U, Alton EWFW (2003) Emerging significance of plasmid DNA nuclear import in gene therapy. Adv Drug Deliv Rev 55:749-760.
• Munter S, Enninga J, Vazques-Martinez R, Delbarre E, David-Watine B, Nehrbass U, Shorte LS (2006) Actin polymerization at the cytoplasmic face of eukaryotic nuclei. BMC Cell Biol 7: 23.
• Ondrej V, Kozubek S, Lukasova E, Falk M, Matula Pa, Matula Pe, Kozubek M (2006) Directional motion of foreign plasmid DNA to nuclear HP1 foci. Chromosome Res 14: 505-514.
• Ondrej V, Lukasova E, Falk M, Kozubek S (2007) The role of actin and microtubule networks in plasmid DNA intracellular trafficking. Acta Biochim Polon 54:657-663.
• Pederson T, Aebi U (2005) Nuclear actin extends with no concentration in sight. Mol Biol Cell 16:5055-5060.
• Porteus MH, Cathomen T, Weitzman MD, Baltimore D (2003) Efficient gene targeting mediated by adeno-associated virus and DNA double-strand breaks. Mol Cell Biol 23: 3558-3565.
• Shumaker DK, Kuczmarski ER, Goldman RD (2003) The nucleoskeleton: lamins and actin are major players in essential nuclear functions. Curr Opin Cell Biol15:358-366.
• Smythe C, Jenkins HE, Hutchinson ChJ (2000) Incorporation of the nuclear pore basket protein Nup 153 into nuclear pore structures is dependent upon lamina assembly: evidence from cell-free extracts of Xenopuseggs. EMBO J 19:3918-3931.
• Verkman AS (2002) Solute and macromolecule diffusion in cellular aqueous compartments. Trends Biochem Sci 27: 27-33.