Purification and characterization of two extracellular lipases from Pseudomonas aeruginosa Ps-x

• Autorzy: Saeed H M , Zaghloul T.I. , Khalil A.I. , Abdelbaeth M.T.
• Języki publikacji: EN

Abstrakty

EN

Two different extracellular lipases were isolated and purified from Pseudomonas aeruginosa Ps-x to apparent homogeneity using ammonium sulfate precipitation followed by ion exchange chromatography on Q- and S-Sepharose column. Both of the purified lipases are monomeric protein with molecular weight of 15.5 and 54.97 KDa respectively. The optimal activities of the enzymes were at 45 and 50°C and pHs 10.0 and 9.0. Calcium ions increase thermostability of both purified lipases I and II. The purified lipase I showed no metal ion dependence for its activity since EDTA up to 10 mM has no effect on the enzyme activity. However purified lipase II showed slight inhibition by EDTA at the same concentration. Moreover, a serine protease inhibitor, PMSF showed an inhibitoiy effect on both purified enzymes.

Słowa kluczowe

EN

lipase; enzyme; Pseudomonas aeruginosa; extracellular lipase; purification; 16S rRNA

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2005
• Tom: 54
• Numer: 3
• Opis fizyczny: p.233-240,fig.,ref.

Twórcy

autor

Saeed H M

• University of Alexandria, Chatby 21526, Alexandria, Egypt

autor

Zaghloul T.I.

autor

Khalil A.I.

autor

Abdelbaeth M.T.

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