Non-genomic effect of ovarian steroids on oxytocin-stimulated prostaglandin [PG] F2alpha and E2 secretion from bovine endometrial cells

• Autorzy: Kowalik M , Kotwica J.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to establish the effect of the ovarian steroids: 17β-oestradiol (E2) and testosterone (T4) on OT-stimulated PGF2α and PGE2 secretion by bovine endometrial cells. The epithelial endometrial cells from days 14-18 of the oestrous cycle (10⁵/ml) were incubated in DMEM/Ham's F12 with 10% FCS (38°C, atmosphere of air and 5% CO₂) for 72-96 h and the last 24 h in DMEM/Ham's F12 with 0.1% BSA. In Exp.l, the doses of steroids used to study their effect on the secretion of PGF2α and PGE2 from endometrial cells stimulated by OT were determined. In Exp.2, the cells were pre-incubated for 30 min with selected doses of steroids: P4 (10⁻⁵ M), T4 (10⁻⁵ M), and E2 (10⁻⁸ M) and next for 4 d with: arachidonic acid (AA; 10⁻⁵ M), OT (10⁻⁷ M) and OT with each of these steroids. The concentration of PGE2 and PGFM -metabolite of PGF2α was determined by EIA. P4, T4, and E2 did not affect (P>0.05) the basal secretion of PGF2α and PGE2, but all the steroids inhibited (P<0.01) OT- stimulated PGF2α secretion. The stimulating effect of OT on PGE2 secretion was not affected by P4 and T4 (P>0.05). This data suggests that different cellular mechanisms exist for steroids affecting the secretion of both prostaglandins from endometrial cells. Moreover, we suggest that non-genomic effect of P4 on bovine endometrial cells is non-specific since the other steroids can impair the effect of OT on these cells. This effect of the steroids can directly modulate function of endometrial cells.

Słowa kluczowe

EN

uterus; cow; endometrium; prostaglandin; progesterone; bovine endometrial cell; reproductive function; steroid; non-genomic effect; female

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2007
• Tom: 51
• Numer: 1
• Opis fizyczny: p.37-42,fig.,ref.

Twórcy

autor

Kowalik M

• Polish Academy of Sciences, 1-=718 Olsztyn-Kortowo, Poland

autor

Kotwica J.

Bibliografia

• 1. Arosh J.A., Banu S.K., Kimmins S., Chapdelaine P., MacLaren L.A., Fortier M.A.: Effect of Interferon-T on prostaglandin biosynthesis, transport, and signaling at the time of maternal recognition of pregnancy in cattle: evidence of polycrine actions of prostaglandin E₂. Endocrinology 2004, 145, 5280-5293.
• 2. Asselin E., Goff A.K., Bergeron H., Fortier M.A.: Influence of sex steroids on the production of prostaglandins F2α, E2, and response to oxytocin in cultured epithelial and stromal cells of the bovine endometrium. Biol Reprod 1996, 54, 371-379.
• 3. Astle S., Khan R.N., Thornton S.: The effects of a progesterone metabolite, 5β-dihydroprogesterone, on oxytocin receptor binding in human myometrial membranes. Br J Obstet Gynaecol 2003, 110, 589-592.
• 4. Bazer F.W., Ott T.L., Spencer T.E.: Endocrinology of the transition from recurring estrous cycles to establishment of pregnancy in sub-primate mammals. In: Bazer F.W., ed. Endocrinology of pregnancy. 1st ed. Totowa N.J., Humana Press, 1998, pp. 1-34.
• 5. Bishop C.V., Stormshak F.: Nongenomic action of progesterone inhibits oxytocin-induced phosphoinositide hydrolysis and prostaglandin F2α secretion in the ovine endometrium. Endocrinology 2006, 147, 937-942.
• 6. Bogacki M., Silvia W.J., Rekawiecki R., Kotwica J.: Direct inhibitory effect of progesterone on oxytocin- induced secretion of prostaglandin F2α from bovine endometrial tissue. Biol Reprod 2002, 67, 184-188.
• 7. Bradford M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72, 248-254.
• 8. Bramley T.: Non-genomic progesterone receptors in the mammalian ovary: some unresolved issues. Reproduction 2003, 125, 3-15.
• 9. Burger K., Fahrenholz F., Gimpl G.: Non-genomic effects of progesterone on the signaling function of G protein- coupled receptors. FEBS Letters 1999, 464, 25-29.
• 10. Burnes P.D., Hayes S.H., Silvia W.J.: Cellular mechanisms by which oxytocin mediates uterine prostaglandin F2α synthesis in bovine endometrium: role of calcium. Domest Anim Endocrinol 1998, 15, 477-487.
• 11. Challis J.R., Sloboda D.M., Alfaidy N., Lye S.J., Gibb W., Patel F.A., Whittle W.L., Newnham J.P.: Prostaglandins and mechanisms of preterm birth. Reproduction 2002, 124, 1-17.
• 12. Dunlap K.A., Stormshak F.: Nongenomic inhibition of oxytocin binding by progesterone in the ovine uterus. Biol Reprod 2004, 70, 65-69.
• 13. Duras M., Mlynarczuk J., Kotwica J.: Non-genomic effect of steroids on oxytocin- stimulated intracellular mobilization of calcium and on prostaglandin F2 and E2 secretion from bovine endometrial cells. Prostagl & Other Lip Med 2005, 76, 105-116.
• 14. Gerdes D., Wehling M., Leube B., Falkenstein E.: Cloning and tissue expression of two putative steroid membrane receptors. Biol Chem 1998, 379, 907-911.
• 15. Gimpl G., Fahrenholz F.: The oxytocin receptor system: structure, function, and regulation. Physiol Rev 2001, 81, 629-683.
• 16. Gimpl G., Fahrenholz F.: Cholesterol as stabilizer of the oxytocin receptor. Biochim Biophys Acta 2002, 1564, 384-392.
• 17. Goff A.K.: Steroid hormone modulation of prostaglandin secretion in the ruminant endometrium during the estrous cycle. Biol Reprod 2004, 71, 11-16.
• 18. Grazzini E., Guillon G., Mouilac B., Zingg H.H.: Inhibition of oxytocin receptor function by direct binding of progesterone. Nature 1998, 392, 509-512.
• 19. Homonics G.E., Silvia W.J.: Effects of progesterone and estradiol-17β on uterine secretion of prostaglandin F2α in response to oxytocin in ovariectomized ewes. Biol Reprod 1988, 38, 723-728.
• 20. Ireland J.J., Murphee R.L., Coulson P.B.: Accuracy of predicting stages of bovine oestrous cycle by gross appearance of the corpus luteum. J Dairy Sci 1980, 63, 155-160.
• 21. Ivell R., Fusch A.R., Bathgate R., Tillmann G., Kimura T.: Regulation of the oxytocin receptor in bovine reproductive tissues and the role of steroids. Reprod Domest Anim 2000, 35, 134-141.
• 22. Kelly M.J., Wagner E.J.: Estrogen modulation of G- protein-coupled receptors. TEM 1999, 10, 369-374.
• 23. Kim J.J., Fortier M.A.: Cell type specificity and protein kinase C dependency on the stimulation of prostaglandin E2 and prostaglandin F production by oxytocin and platelet-activating factor in bovine endometrial cells. J Reprod Fert 1995, 103, 239-247.
• 24. Klein U., Gimpl G., Fahrenholz F.: Alteration of the myometrial plasma membrane cholesterol content with β- cyclodextrin modulates the binding affinity of the oxytocin receptor. Biochemistry 1995, 34, 13784-13793.
• 25. Kotwica J., Skarzynski D.: Influence of oxytocin removal from corpus luteum on secretory function and duration of the oestrous cycle in cattle. J Reprod Fertil 1993, 97, 411-417.
• 26. Kotwica J., Skarzynski D., Bogacki M., Melin P., Starostka B.: The use of an oxytocin antagonist to study the function of ovarian oxytocin during luteolysis in cattle. Theriogenology 1997, 48, 1287-1299.
• 27. Kotwica J., Skarzynski D., Miszkiel G., Melin P., Okuda K.: Oxytocin modulates the pulsatile secretion of prostaglandin F2α in initiated luteolysis in cattle. Res Vet Sci 1999, 66, 1-5.
• 28. Lafrance M., Goff A.K.: Effects of progesterone and estradiol-17β on oxytocin-induced release of prostaglandin F2α in heifer. J Reprod Fertil 1988, 82, 429-436.
• 29. Lamming G.E., Mann G.E.: Control of endometrial oxytocin receptors and prostaglandin F2 production in cows by progesterone and estradiol. J Reprod Fert 1995, 103, 69-73.
• 30. Machelon V., Nome E., Grosse B., Lieberherr M..: Progesterone triggers rapid transmembrane calcium influx and/or calcium mobilization from endoplasmic reticulum, via a pertussis-insensitive G-protein in granulosa cells in relation to luteinization process. J Cell Biochem 1996, 61, 619-628.
• 31. McCracken J.A., Custer E.E., Lamsa J.C.: Luteolysis: a neuroendocrine-mediated event. Physiol Rev 1999, 79, 263-323.
• 32. Młynarczuk J., Sasiadek J., Kotwica J.: Non genomie action of progesterone in luteal and endometrial epithelial cells in cattle. Bull Inst Vet Pulawy 2005, 49, 193-198.
• 33. Moss R.L., Gu Q., Wong M.: Estrogen: nontranscriptional signaling pathway. Recent Prog Horm Res 1997, 52, 33-69.
• 34. Niswender G.D., Juengel J.L., Silvia P.J., Rollyson M.K., McIntush E.W. Mechanism controlling the function and life span of the corpus luteum. Physiol Rev 2000, 80, 1- 29.
• 35. Peluso J.J.: Multiplicity of progesterone's actions and receptors in the mammalian ovary. Biol Reprod 2006, 75, 2-8.
• 36. Peluso J.J., Fernandez G., Pappalardo A., White B.A.: Characterization of a putative membrane receptor for progesterone in rat granulose cells. Biol Reprod 2001, 65, 94-101.
• 37. Pietras R.J., Szego C.M.: Specific binding sites for oestrogen at the outer surfaces of isolated endometrial cells. Nature 1977, 265, 69-72.
• 38. Pietras R.J., Szego C.M.: Partial purification and characterization of oestrogen receptors in subfractions of hepatocyte plasma membranes. Bioch Journal 1980, 191, 743-760.
• 39. Poyser N.L. The control of prostaglandin production by the endometrium in relation to luteolysis and menstruation. Prostaglandins Leukot Essent Fatty Acids 1995, 53, 147-195.
• 40. Pratt B.R., Butcher R.L., Inskeep E.K.: Antiluteolytic effect of the conceptus and of PGE2 in ewes. J Anim Sci 1995, 45, 784-791.
• 41. Rae M.T., Menzies G.S., Bramley T.A.: Bovine ovarian non-genomic progesterone binding sites: presence in follicular and luteal cell membranes. J Endocrinol 1998, 159, 413-427.
• 42. Rae M.T., Menzies G.S., McNeilly A.S., Woad K., Webb R., Bramley T.A.: Specific non-genomic, membrane- localized binding sites for progesterone in the bovine corpus luteum. Biol Reprod 1998, 58, 1394-1406.
• 43. Ramirez V.D., Zheng J., Sidique K.M.: Membrane receptors for estrogen, progesterone, and testosterone in the rat brain: fantasy or reality. Cell Mol Neurobiol 1996, 16, 175-197.
• 44. Revelli A., Massobrio M., Tesarik J.: Nongenomic action of steroid hormones in reproductive tissues. Endocr Rev 1998, 19, 3-17.
• 45. Silvia W.J., Lewis G.S., McCracken J.A., Thatcher W.W., Wilson J.R.: Hormonal regulation of uterine secretion of prostaglandin F2α during luteolysis in ruminants. Biol Reprod 1991, 45, 655-663.
• 46. Simoncini T., Genazzani A.R.: Non-genomic actions of sex steroid hormones. Eur J Endocrinol 2003, 148, 281-292.
• 47. Skarzynski D.J., Bogacki M., Kotwica J.: Involvement of ovarian steroids in basal and oxytocin-stimulated prostaglandin (PG)F2 secretion by the bovine endometrium in vitro. Theriogenology 1999, 52, 385- 397.
• 48. Skarzynski D.J., Miyamoto Y., Okuda K..: Production of prostaglandin F2α by cultured bovine endometrial cells in response to tumor necrosis factor α: cell type specificity and intracellular mechanisms. Biol Reprod 2000, 62, 1116-1120.
• 49. Stormshak F.: Biochemical and endocrine aspects of oxytocin production by the mammalian corpus lutenm. Reprod Biol Endocrinol 2003, 1, 92.
• 50. Thatcher W.W., Guzeloglu A., Mattos R., Binelli M., Hansen T.R., Pru J.K.: Uterine-conceptus interactions and reproductive failure in cattle. Theriogenology 2001, 56, 1435-1450.
• 51. Wehling M.: Specific, nongenomic actions of steroid hormones. Annu Rev Physiol 1997, 59, 365-393.
• 52. Xiao C.W., Liu J.M., Sirois J., Goff A.K.: Regulation of cyclooxygenase-2 and prostaglandin F synthase gene expression by steroid hormones and interferon-τ in bovine endometrial cells. Endocrinology 1998, 139, 2293-2299.