The heterogeneity of cell subtypes from a primary culture of human amniotic fluid

• Autorzy: Zhang S , Geng H. , Xie H. , Wu Q. , Ma X. , Zhou J. , Chen F.
• Języki publikacji: EN

Abstrakty

EN

Heterogeneous human amniotic fluid contains various cell types. Herein, we report on the possibility of simultaneously isolating three subtypes of cells from one primary culture. Using a stainless steel instrument named a colony poculum, two of the three cell subtypes could be efficiently cultured, and these were further characterized. The results indicated that these two cell subtypes had different morphologies and were characterized by different cell marker expression profiles, including the differential expression of CD105, CD117 and EBAF. Furthermore, their gene expression array data revealed their different gene expression profiles. Although both cell types expressed several embryonic stem cell-specific markers, they were non-tumorigenic in vivo. This paper not only provides new insight into the heterogeneity of human amniotic fluid, it also presents a simple yet efficient cell isolation method. These results will contribute to the thorough investigation of the properties and potential future applications of human amniotic fluid-derived cells.

Słowa kluczowe

EN

heterogeneity; cell subtype; primary culture; man; amniotic fluid; gene expression; colony poculum

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2010
• Tom: 15
• Numer: 3
• Opis fizyczny: p.424-439,fig.,ref.

Twórcy

autor

Zhang S

• Shanghai Jiao Tong University School of Medicine, Shanghai, China

autor

Geng H.

autor

Xie H.

autor

Wu Q.

autor

Ma X.

autor

Zhou J.

autor

Chen F.

Bibliografia

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