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2006 | 28 | 3 |

Tytuł artykułu

Studies on the superoxide anion production and peroxidase activity in potato leaf cell suspension exposed to salicylic acid

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
It is now widely accepted that salicylic acid (SA) signaling is mediated by reactive oxygen species (ROS) production. We have studied the effect of SA on peroxidase activity and superoxide anion production in potato leaf cell suspension. The results show that potato cells are insensitive to low concentrations of exogenous SA (< 1 mM) and the effect is observed at 1-5 mM SA. The cells exposed to SA exhibit higher peroxidase activity and show different peroxidase pattern when antiyzed on native gels compared to the control. Superoxide anion production is enhanced after two hours of treatment and 2.5 mM SA gives the highest value. The results suggest peroxidase-mediated detoxification of ROS elicited by SA.

Wydawca

-

Rocznik

Tom

28

Numer

3

Opis fizyczny

p.237-243,fig.,ref.

Twórcy

autor
  • University of Tehran, Tehran, Iran
autor

Bibliografia

  • Abeles F. B., Biles C. L. 1991. Characterization of peroxidase in lignitytng peach fruit Endocrop. Plant Physiol. 95: 269-273.
  • Alvarez M. E. 2000. Salicylic acid in the machinery of hypersensitive cell death and disease resistance . Plant Mol. Biol. 44: 429-442.
  • Bolwell G. P. 1999. Role of active oxygen species and NO in plant defense responses. Curr. Opin. Plant Biol. 2: 287-294.
  • Bradford M. M. 1976. A rapid and sensitive methods for the quantification of microgram quantities of protein utitiztng the principle of protein dye bindtng . Anal. Biochem. 72: 248-254.
  • Coligan J. E., Kruisbeek A. M., Margulies D. H., Shevach E. M., Strober W. 1991. Current Protocols in Immunol. Unit 3.17, Wiley, New York.
  • Delaney T.P., Uknes S., Vernooij B., Friedrich L., Weymann K., Negrotto D., Gaffney T., Gul-Rella M., Kessmann H., Ward E., Ryals J. 1994. Central role of salicylic acid in plant defense resistance. Science 266: 1247-1250.
  • Dixon R. A. 2001. Natural products and plant disease resistance. Nature, 411: 843-847.
  • Enyedi A. J., Yalpani N., Silverman P., Raskin I 1992. Localization, conjugation and function of salicylic acid in tobacco during the hypersensitive reaction to tobacco mosaic virus. Proc. Natl. Acad. Sci. U.S.A 89: 2480-2484.
  • Hames B. D., Rickwood D. 1999. Gel electrophoresis of protein, a practical approach. Oxford University Press. PP. 1-138.
  • Heath C. M. 2000. Hypersensitive response-related death . Plant Mol. Biol. 44: 321-334.
  • Hiraga S., Sasaki K., Ito H., Ohashi Y., Matsui H. 2001. A large family of class III plant peroxidases. Plant Cell Physiol. 42: 462-468.
  • Huh G. H., Lee S. J., Bae Y. S., Liu J. R., Kwak S. S. 1997. Molecular cloning and characterization of cDNAs for anionic and neutral peroxidases from suspension cultured cells of sweet potato and their differential expression in response to stress. Mol. Genet. 255: 382-391.
  • Johnson S. M., Doherty S. J., Croy R. R. D. 2003. Biphasic superoxide generation in potato tubers, a self-amplifying response to stress . Plant Physiol. 131: 1440-1449.
  • Kawano T., and Muto S. 2000. Mechanism of peroxidase actions for salicylic acid induced generation of active oxygen species and an increase in cytosolic calcium in tobacco cell suspension culture. J. Exp. Bot. 51: 685-693.
  • Kawano T. 2003. Roles of the reactive oxygen spe t cies-generating peroxidase reactions in plant defense and growth induction . Plant Cell Reports 21: 829-837.
  • Larkindale J., Knight M. R. 2002. Protection against heat stress-induced oxidative damage in Arabidopsis involved calcium abscisic acid, ethylene and salicylic acid. Plant Physiol. 128: 682-695.
  • Maxwell D. P., Wang Y., Macintosh L. 1999. The alternative oxidase lowers mitochondria reactive oxygen production in plant cells. Proc. Natl. Acad. Sci. U.S.A. 9: 8271-8276.
  • Mayo L. A., Currutle J. T. 1990. Kinetic Micro plate assay for superoxide production by Neutrophil and other phagocytic cells. Methods Enzymol. 186: 567-574.
  • Minibayeva F., Mika A., Luthje S. 2003. Salicylic acid changes the properties of extracellular peroxidase activity secreted from wounded wheat roots. Protoplasma 221: 67-72.
  • Minibayeva F., Beckett R.P. 2001. High rates of extracellular superoxide production in bryophytes and lichens and an oxidative burst in response to dehydration following desiccation. New Phytol. 152: 333-343.
  • Murashige T., Skoog F. 1969. A revised for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant., 15: 473-497.
  • Naton B., Hahlbrock K., Schmelzer E. 1996. Correlation of rapid cell death with metabolic changes in fungus-infected, cultured parsley cells. Plant Physiol. 112: 433-444.
  • Rao M.V., Paliyath G., Ormrod D. P., Murr D.P., Watkins C. B. 1997. Influence of salicylic acid on H2O2 production, oxidative stress, and H2O2-metabolizing enzymes. Plant Physiol. 115: 137-149.
  • Raskin I. 1992. Role of sahcylic acid in plants. Ann. Rev. Plant Physiol. Plant Mol. Biol. 43 : 439-463.
  • Rusterucci C., Stallaert V., Milat M. L., Pugin A., Ricci P., Blein J. P. 1996. Relation between active oxygen species, lipid peroxidation, necros is and phytoalexin production by elicitors in Nicotiana. Plant Physiol. 111: 885-891.
  • Sakhabutdinova A. R., Farkhutdinova D. R., Bezrukova M. V., Shakirova F. M. 2003. Salicylic acid prevents the damagmg action of stress factors on wheat plants. Bulg. J. Plant Physiol., Special Is tue 314-319.
  • Stich K., Ebermann R. 1988. Investigation of substrate especially of peroxidase isoenzymes, occurring in wood of different species . Hozgorschung 42: 221-224.
  • Thulke O., Conrath U. 1998. Salicylic acid has a dual role in the activation of defense related genes in parsley. The Plant J. 14: 35-42.
  • Tian H. R., Zhang G. Y., Yan C. H., Dai Y. R. 2000. Involvement of poly (ADP-ribose) polymerase and activation of caspase-3-like protease in heat shock-induced Apoptosis in tobacco suspension cells. FEBS Lett. 474: 11-15.
  • Yoshioka K., Kachroo P., Tsui F., Sharma S. B., Shah J., Klessing D. F. 2001. Environmental sensitive SA-dependent defense responses in the CPr22 mutant of Arabidopsis. The Plant J. 26: 447-459.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-80124380-4825-428d-9cda-aff925065dc5
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