Humoralna odpowiedz na wybrane antygeny paleczek Yersinia enterocolitica i Yersinia pseudotuberculosis w przebiegu jersiniozy u ludzi. II. Wystepowanie przeciwcial dla wydzielniczych bialek Yop wykrytych odczynem western-immunoblotting

• Autorzy: Rastawicki W.

Warianty tytułu

EN

Humoral response to selected antigens of Yersinia enterocolitica and Yersinia pseudotuberculosis in the course of yersiniosis in humans II. Occurrence of antibodies to Yersinia Yop proteins by western-blot

• Języki publikacji: PL

Abstrakty

PL

Oceniono odczynem western-immunoblotting częstość występowania przeciwciał dla poszczególnych wydzielniczych białek Yop w próbkach surowicy osób z różnymi postaciami jersiniozy. Najczęściej w próbkach surowicy osób z objawami bólów brzucha, zapalenia stawów i rumienią guzowatego wykrywano przeciwciała dla białka YopD o masie 36 kDa. Przeciwciała klasy IgG dla białek YopD, YopM i YopH występowały w podobnym odsetku u osób z objawami bólów brzucha i zapaleniem stawów, przeciwciała klasy IgA dla tych białek dominowały u osób z zapaleniem stawów natomiast przeciwciała klasy IgM u osób z objawami bólami brzucha.

EN

The results obtained with the use of the western-blotting showed that antibodies for released proteins YopD (33-36 kDa) were the most frequently detected antibodies in serum samples from patients suspected for yersiniosis. Reactions between serum samples studied and the YopD proteinwere very intense, suggesting that protein is the strongest immunogen among the utilised, released proteins Yop of Yersinia. Antibodies IgM were more often diagnosed in patients with abdominal pain in the contrary to antibodies IgA which were characteristic to patients with reactive arthritis. Detailed analysis of the results of western-blotting on serum samples obtained several times from individuals with yersinosis during the course of infection in this investigation have showed also that antibodies of the IgA class hold longer in serum of individuals with arthritis compared with individuals with yersinosis not complicated by arthritis. In joint-fluid samples obtained from patients with arthritis antibodies for particular released proteins Yop were detected in the same class of immunoglobulins like in serum samples obtained from those individuals.

Słowa kluczowe

PL

test Western-blot zob.metoda Western blot; test Western-immunoblotting zob.metoda Western blot; metoda Western blot; choroby czlowieka; jersynioza; antygeny; Yersinia enterocolitica; Yersinia pseudotuberculosis; odpowiedz humoralna; przebieg choroby; przeciwciala; wystepowanie; bialko Yop; diagnostyka serologiczna; zapalenie stawow; bole brzucha

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2006
• Tom: 58
• Numer: 4
• Opis fizyczny: s.321-328,rys.,tab.,bibliogr.

Twórcy

autor

Rastawicki W.

• Panstwowy Zaklad Higieny w Warszawie, ul.Chocimska 24, 00-791 Warszawa

Bibliografia

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